Introduction:Owing to its favorable radionuclidic characteristics, such as tl/2 = 6.73 day and Eβ (max) = 497 keV and ease of its large-scale production using medium flux research reactors, lutetium-177 (177Lu) is an attractive radionuclide for various therapeutic applications. No carrier added (NCA) 177Lu was obtained by thermal neut...

Size exclusion chromatography (SEC) has been widely applied in protein separation based on their molecular sizes. Here we present a peptide mixture from 1kD to 6 kD separation on size exclusion chromatography column Zenix TM -80. The effect of different mobile phases on the peptides’ separation performances was also explored. In the separation of insulin, glucagon, angiotensin I and bradykinin,...

Reversed-phase chromatography is a method that is often used for glycan separation. For this, glycans are often derivatized with a hydrophobic tag to achieve retention on hydrophobic stationary phases. The separation and elution order of glycans in reversed-phase chromatography is highly dependent on the hydrophobicity of the tag and the contribution of the glycan itself to the retention. The c...

499: 177. Lindner H, Sarg B and Helliger W (1997) Application of hydrophilic-interaction liquid chromatography to the separation of phosphorylated H1 histones. Journal of Chromatography 728: 55. Mant CT and Hodges RS (eds) (1991) High-performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis, and Conformation. Boca Raton: CRC Press. Mant CT and Hodges RS (1996) Analysis o...

In proteomics, nanoflow multidimensional chromatography is now the gold standard for the separation of complex mixtures of peptides as generated by in-solution digestion of whole-cell lysates. Ideally, the different stationary phases used in multidimensional chromatography should provide orthogonal separation characteristics. For this reason, the combination of strong cation exchange chromatogr...

The high-pressure liquid chromatographic separation of ail of the known metabolites of vitamin D2 and vitamin D3 found in biological fluids has been achieved. This technique has been successfully applied to the analysis of vitamin D mixtures, purification of vitamin D metabolites, and identification of radioactive peaks. Some theoretical bases for the observed resolutions are suggested. Supplem...

two dimensional thin layer chromatography on polyamide sheets is a useful method for identification of pth-amino acids which have very close rf-values. the solvent systems described here are particularly effective and have an edge over other solvent systems used for the separation of pth-amino acids. the solvent systems provide a clear separation of all pth-amono acids except lle / leu in a rel...

Было изучено хроматографическое поведение гидроксиантрахинонов. Хорошее разделение было достигнуто при использовании предложенных новых хроматографических систем: бумажная хроматография смесью уксусной кислоты и воды на простой бумаге или бумаге импрегнированной оливковым мас­ лом, тонкослойная хроматография на целлюлозе импрегнированной диметилформамидом и на силикагеле без или с импрегнацией ...