The interactions between pig heart citrate synthase and mitochondrial malate dehydrogenase or cytosolic malate dehydrogenase were studied using the frontal analysis method of gel filtration and by precipitation in polyethylene glycol. This method showed that an interaction between citrate synthase and mitochondrial malate dehydrogenase occurred but no interaction between citrate synthase and cy...

A method for determining citrate as its complex with iron(II1) by differential pulse polarography is described. The method is sensitive to 5 pg ml-1 of citrate, without any interference from equimolar propan-2-onedicarboxylate and propenetricarboxylate ions and 10% of oxalate and tartrate ions. The limits of error in citrate concentrations determined by the proposed method are better than &2.6y...

BACKGROUND AND AIMS Aluminium (Al) stimulates the efflux of citrate from apices of rice bean (Vigna umbellata) roots. This response is delayed at least 3 h when roots are exposed to 50 microm Al, indicating that some inducible processes leading to citrate efflux are involved. The physiological bases responsible for the delayed response were examined here. METHODS The effects of several antago...

objective: dendrimers are three-dimensional nanostructures that have numerous applications in medicine, including drug delivery and imaging. although anionic dendrimer polyethylene glycol–citrate has a high potential to increase solubility of water-insoluble drugs and drug delivery, its multi-step synthesis procedure is time consuming. in addition, toxic substances such as dichloromethane are u...

In the work it is shown by spectrophotometry method that depending on concentration ratio of ligands [PPi4–]/[Cit3–] in pyrophosphate-citrate electrolyte, cobalt (II) ions form not only citrate [Co(Cit)2]4– and pyrophosphate [Co(PPi)2]6–, but also polyligand complexes [Co(PPi)m(Cit)n]+2–(4m+3n). The composition [Co(PPi)Cit]5– was determined, equilibrium constant reaction their formation stabili...

Citrate synthetase activity was determined in samples of commercially available citrate condensing enzyme. We measured the amount of radioactive citrate formed from the condensation of carbon-14 labeled acetyl CoA and oxaloacetate. The labeled citrate was oxidized by oxidative bromination; 14C02 evolved was collected and represented the amount of citrate synthesized. Enzyme activity as low as 1...

A kit method for rapid analysis of citrate in foodstuffs, based on use of citrate lyase (EC 4.1.3.6), has been adapted and evaluated for use with clinical samples. Membrane filters removed serum proteins, which interfere with the analysis, and reagent and sample volumes were decreased from those in the manufacturer's protocol, thereby decreasing the cost of the assay. Using gravimetrically prep...

High incidence of multiple kidney stone formation has been observed among workers exposed to cadmium (Cd). Citrate is known to be a protective factor against renal stone formation. To study the direct effect of cadmium on citrate uptake by the renal brush border membrane, we exposed isolated rat renal brush border membrane vesicles (BBMV) to cadmium and determined their citrate uptake character...