In Bangladesh, citrus is an important fruit crop that becoming more commonly cultivated in commercial plantations. March 2019, we observed yellowing, vein clearing, and mild mosaic symptoms citron (Citrus medica). Based on systematic serological molecular analyses of the symptomatic leaf samples, Citrus tristeza virus (CTV) was identified. To best our knowledge, this first report CTV any Bangla...

Citrus tristeza is one of the most destructive citrus diseases and is caused by the phloem-restricted Closterovirus, Citrus tristeza virus. Mild strain CTV-B2 does not cause obvious symptoms on indicators whereas severe strain CTV-B6 causes symptoms, including stem pitting, cupping, yellowing, and stiffening of leaves, and vein corking. Our laboratory has previously characterized changes in tra...

background and aims: plant certification programs need reliable, fast, cheap and sensitive methods for detection of systemic pathogens with special interest in virus and viroid detection. reverse transcriptase-polymerase chain reaction (rt-pcr) has been documented as an alternative assay for certification of plant propagating materials. the main object of the present study was the optimization ...

BAR-JOSEPH, M., S. M. GARNSEY, D. GONSALVES, M. MOSCOVITZ, D. E. PURCIFULL, M. F. CLARK, and G. LOEBENSTEIN. 1979. The use of enzyme-linked immunosorbent assay for detection of citrus tristeza virus. Phytopathology 69:190-194. An enzyme-linked immunosorbent assay (ELISA) test was used to seedling yellows isolates of CTV from Israel and from Florida. Isolates that identify citrus tristeza virus ...

tristeza caused by citrus tristeza virus (ctv), is the most important viral disease of citrus worldwide. serious damages of the disease have been reported from many citrus growing areas of the world. ctv particles are restricted to phloem and are transmitted by aphids in a semipersistent manner. ctv was introduced from japan to mahdasht orchards in mazadaran province in 1969. its natural spread...

Systemic invasion of plants by viruses is thought to involve two processes: cell-to-cell movement between adjacent cells and long-distance movement that allows the virus to rapidly move through sieve elements and unload at the growing parts of the plant. There is a continuum of proportions of these processes that determines the degrees of systemic infection of different plants by different viru...

The Citrus tristeza virus (CTV) strains present in an area can determine the type and severity of disease produced. Using real time RT-qPCR, we screened a series of representative samples collected throughout Florida from 1964 to 2002 for CTV strain presence. We found that contrary to previous reports, the historical samples were infected with both the T30 and T36 strains, the latter often at l...

In nature Citrus tristeza virus (CTV), genus Closterovirus, infects only the phloem cells of species of Citrus and related genera. Finding that the CTV T36 strain replicated in Nicotiana benthamiana (NB) protoplasts and produced normal virions allowed development of the first genetic system based on protoplast transfection with RNA transcribed from a full-genome cDNA clone, a laborious and unce...

The control of Citrus tristeza virus (CTV), the causal agent of tristeza disease, is essential to guarantee a productive citrus industry. The specific features of a nursery block make the control of the natural spread of viruses transmitted by vectors more difficult. Thus, the knowledge of the epidemiology of CTV in nursery blocks is basic to the design control strategies of the disease. Two ex...

The large RNA genome of Citrus tristeza virus (CTV; ca. 20 kb) contains 12 open reading frames, with the 3'-terminal one corresponding to a protein of 209 amino acids (p23) that is expressed from an abundant subgenomic RNA. p23, an RNA-binding protein with a putative zinc-finger domain and some basic motifs, is unique to CTV because no homologs have been found in other closteroviruses, includin...