Members of the actinomycete genus Clavibacter are known to produce antimicrobial compounds, but so far none of these compounds has been purified and characterized. We have isolated an antimicrobial peptide, michiganin A, from the tomato pathogen Clavibacter michiganensis subsp. michiganensis, using ammonium sulfate precipitation followed by cation-exchange and reversed-phase chromatography step...

We report here the complete genome sequence of Clavibacter michiganensis subsp. insidiosus R1-1, isolated in Minnesota, USA. The R1-1 genome, generated by a de novo assembly of PacBio sequencing data, is the first complete genome sequence available for this subspecies.

Endophytic bacteria were isolated from the stalks of sugarcane, Saccharum officinarum, growing in Papua New Guinea. Endophytic bacterial communities from extracted juice averaged 6x 10 cfu/ml, which is 25 times greater than the number of endophytic bacteria found in commercial varieties in South Africa. Sixty isolates, representing the different colony morphology types, were used for in vitro i...

Contour-clamped homogeneous electric field gel analysis of genomic DNA of the plant pathogen Clavibacter michiganensis subsp. sepedonicus revealed the presence of a previously unreported extrachromosomal element. This new element was demonstrated to be a linear plasmid. Of 11 strains evaluated, all contained either a 90-kb (pCSL1) or a 140-kb (pCSL2) linear plasmid.

The molecular interactions between Clavibacter michiganensis subsp. michiganensis and tomato plant were studied by following the expression of bacterial virulence and host-defense genes during early stages of infection. The C. michiganensis subsp. michiganensis genes included the plasmid-borne cellulase (celA) and the serine protease (pat-1), and the serine proteases chpC and ppaA, residing on ...

To enhance plant protection against phytopathogens, we constructed a fusion gene (SAP) for the simultaneous production of snakin-1 (SN1) and defensin-1 (PTH1) cysteine-rich antimicrobial proteins originally identified in potato. Prior to in vivo evaluation of antimicrobial activity, SAP produced in Escherichia coli was tested in in vitro against four phytopathogenic microorganisms. SAP exhibite...

The quarantine bacterium Clavibacter michiganensis subsp. sepedonicus (Cms) causes bacterial ring rot (BRR) in potato but is difficult to detect, hampering the diagnosis of this disease. ELISA immunoassays have not been widely used to detect Cms because commercially available anti-Cms antibodies detect mainly EPS-producing bacteria and can fail to detect strains that do not produce EPS. In the ...