Background and Aims: Rotaviruses are one of the main causative agents of acute viral gastroenteritis in both young human and animals. Several methods have been used for diagnosis of rotavirus infection. Most of these methods are not easily available in Iran. Methods: In our study attempt was made to develop a method which could replace the commercially imported Kit. Rota virus was purified and...

Rates of detection of rotavirus were compared by diagnostic assay and specimen type. For bulk stools, rates of detection by reverse transcriptase PCR (RT-PCR) and enzyme immunoassay (EIA) were similar, but 18% of healthy controls tested positive by RT-PCR. Testing of bulk stools by EIA appears to be optimum for rotavirus surveillance.

BACKGROUND Listeriosis is an opportunistic bacterial infection caused by Listeria monocytogenes and predominantly affects people who are immunocompromised. Due to its severity and the population at risk, prompt clinical diagnosis and treatment of listeriosis is essential. A major step to making a clinical diagnosis is the collection of the appropriate specimen(s) for testing. This study explore...

A 44-year-old woman developed Legionella pneumophila pneumonia after cerebral surgery. Initially, one colony from a clinical specimen and two colonies from water samples, all belonging to serogroup 12, did not match when their DNA restriction patterns were compared. When additional colonies from the water specimens were analyzed, a serogroup 12 strain complementary to that found in the clinical...

Peptococcus indolicus was isolated from a skin lesion of a sheepherder. A case report is given, and microbiological features of this organism are described.