Production of toxins by Clostridium septicum and Clostridium chauvoei has been the subject of many conflicting reports. Roux and Chamberland (1887) first noted that 3 to 4 day filtrates were antigenic; conferring on guinea pigs an immunity against infection with viable organisms. Production of a lethal toxin by C. septicum was however, demonstrated by Leclainche (1898) and Leclainche and Morel ...

Monoclonal antibodies to flagella of Clostridium chauvoei were obtained by the fusion of murine myeloma cells (P3-X63-Ag8-U1) and spleen cells from BALB/c mice immunized with partially purified flagella of strain Okinawa. Enzyme-linked immunosorbent assay and Western blot analysis with partially purified flagella, flagellated cells, and nonflagellated mutants were used to show that five monoclo...

Specific antiflagellar serum of Clostridium chauvoei showed a powerful protective effect which prevented bacterial growth in the liver, but not in infected muscle, against intramuscular challenge with calcium chloride-activated spores in normal mice. No protective effect was observed in mice with polymorphonuclear leucocytes depleted by cyclophosphamide treatment. The antiflagellar serum had ap...

The objective of the present study was to isolate and identify Clostridium chauvoei from swab (n=750) and tissue (n=50) samples from cattle suspected for black quarter in six districts belonging to different climatic zones (Punjab, Pakistan). Conventional culture methods showed 192(25.6%) and 19(38%) positive from swab and tissues respectively for this bacterium. These bacterial isolates were f...

Clostridium chauvoei is the causative agent of blackleg, a wide spread serious infection of cattle and sheep with high mortality. In this study we have analyzed the sialidase activity of the NanA protein of C. chauvoei and cloned the sialidase gene nanA. Sialidase is encoded as a precursor protein of 722 amino acids with a 26 amino acid signal peptide. The mature sialidase has a calculated mole...

Full genome sequences of 20 strains of Clostridium chauvoei, the etiological agent of blackleg of cattle and sheep, isolated from four different continents over a period of 64 years (1951-2015) were determined and analyzed. The study reveals that the genome of the species C. chauvoei is highly homogeneous compared to the closely related species C. perfringens, a widespread pathogen that affects...

  Blackleg in cattle has been recognized since 1938 in Iran that mostly affects cattle in enzootic farm. Main object of this study was to prepare and formulate a concentrated potent vaccine for immunization of cattle against blackleg in Iran. Experimental concentrated blackleg vaccine was prepared according to the method described by FAO. The medium (Modified medium for production of experiment...