A substantial amount of cytochrome oxidase subunit III (COIII) mRNA continues to be synthesized de novo in Trypanosoma brucei in the presence of actinomycin D, presumably by a DNA-independent transcription process. We describe the identification of negative-strand COIII RNA molecules, characterization of their termini, and the detection of RNA-dependent RNA polymerase activity. Three lines of e...

Partially edited mRNAs were selected by the polymerase chain reaction and sequenced. In the case of cytochrome b, 102 out of 106 clones displayed patterns of editing that were consistent with a strictly progressive 3' to 5' editing process, as predicted by the guide RNA model of RNA editing. In the case of cytochrome oxidase subunit III (COIII), 177 out of 304 clones displayed strictly progress...

By employing the HSAB principle and “assisted self-assembly” approach using 2-pyridylaldoximate (pao−) as primary ligand pivalate (piv−) ancillary co-ligand, tetranuclear [CoIII2LnIII2(NO3)4(pao)4(piv)4] complex polynuclear compounds were isolated (Ln = Dy, Gd, Tb, Pr, Y). The structure of Dy(III) was determined via single-crystal X-ray crystallography, revealing a metal topology two {CoIIIDyII...

The design of advanced metal–organic framework (MOF) catalysts for solar-driven conversion CO2 into syngas (CO/H2 mixture) is beneficial. Herein, the a joint MOF heterostructure consisting orderly assembled CoII- and CoIII-based Prussian blue analogs (PBAs) driven by their spontaneous lattice match in growth process reported. As-prepared H/CoIII[email protected]II-PBA cage mesocrystal exhibits ...

Photocatalytic Syngas Production In article number 2200085, Mingxiong Lin, Zanyong Zhuang, Yan Yu, and co-workers report the construction of an ordered CoIII[email protected]II-MOF heterostructure based on a spontaneous oriented attachment mechanism. CoIII-PBA exerts stronger affinity for CO2 molecules than CoII-PBA, thus serves as active sites, built-in electric field in protected]II-PBA heter...

Guide RNAs (gRNAs) for the editing of sites 1-8 of COIII mRNA and an "unexpected" partially edited COIII mRNA are encoded in the variable regions of specific kinetoplast DNA minicircles. The gRNAs can form 37 and 44 nucleotide perfect hybrids (allowing for G-U base pairs) with edited mRNAs. The gRNAs were detected on Northern blots and shown to have unique 5' ends situated close to the beginnin...

The carcinogenicity of estrogens in rodents and man has been attributed to either alkylation of cellular macromolecules and/or redox-cycling, generation of active radicals and DNA damage. Metabolic activation of estradiol leading to the formation of catechol estrogens is believed to be a prerequisite for its genotoxic effects. 4-Hydroxyestradiol is a potent inducer of tumors in hamsters. Previo...

Although less famous than low-spin trivalent cobalt found in [CoIII(NH3)6?xClx]Cl3?x (x ? 3), which was exploited by Alfred Werner during the early part of 20th century for establishing basic rules coordination chemistry, related chromium complexes exhibit comparable kinetic inertness, a rare property along 3d-transition series. The associated slow isomerisation processes are compatible with is...