Background & Aims: Induced pluripotent stem cells (iPSCs) have the capability to undergo unlimited selfrenewal and differentiation into all cell types in the body. These cells are artificially derived from a nonpluripotent cell, typically human dermal fibroblasts (HDFs). The study of cytogenetic stability of these cells, in order to use iPS cells and apply studies in therapeutic applications, i...

Background: Occupational health is a sensitive field in many countries where occupational exposure needs attention due to the lack of awareness. Several businesses, including leather, textile, iron, steel, and dyeing industries use a variety of mutagenic and genotoxic chemicals. Exposure to these chemicals may pose serious health risks, including genetic damage to workers. Comet assay is a tech...

Although the Comet assay, a procedure for quantitating DNA damage in mammalian cells, is considered sensitive, it has never been ascertained that its sensitivity is higher than the sensitivity of other genotoxicity assays in mammalian cells. To determine whether the power of the Comet assay to detect a low level of genotoxic potential is superior to those of other genotoxicity assays in mammali...

background: organophosphorus pesticides has been widely used in agriculture fields to control various crop insects and their extensive use pose human life at threat because of their adverse effects on human health. in this study, we checked the effects of gst polymorphism on ameliorative effect of curcumin and carvacrol against dna damages. methods: comet assay was used to assess the dna damage...

background: various frequencies of sperm aneuploidy are reported in sperms of subfertile patients compared to normal individuals. moreover, sperm dna damage is shown to be associated with male infertility. in this study, the rate of dna damage and frequencies of aneuploidy in sperms of subfertile patients was investigated. methods: semen samples were obtained from healthy normal and subfertile ...

objective: irinotecan is a botanical derivative and an anti-cancer drug with cytotoxic and genotoxic effects. the present study evaluated the effect of lactobacillus plantarum a7 on the genotoxic activity of irinotecan in a hepatocellular carcinoma cell line (hepg2) by comet assay. materials and methods: hepg2 were incubated with irinotecan (100 µm), heat-killed cells (0.025 µg/ml) + irinotecan...

objective: ferula szowitsiana has been widely used for medicinal purposes around the world. the anti-oxidant effect of f.szowitsiana had been proved. the current study aims to determine the protective effects of galbanic acid, a sesquiterpene coumarin from f. szowitsiana, against hydrogen peroxide (h2o2) - induced oxidative dna damage in human lymphocytes. materials and methods: human lymphocyt...

background: ˝radioadaptive response˝ is well-documented phenomenon appeared in low dose ionizing radiation received in vitro and in vivo. occupational exposure has always been a great concern for radiation workers therefore this study was performed to study radioadaptive response in terms of residual dna double strand breaks as an endpoint in peripheral blood leukocytes of occupationally expose...