Two methods of performing serological screening tests for syphilis are compared. One consisted of the Venereal Diseases Reference Laboratory (VDRL) slide test, the cardiolipin Wassermann reaction (CWR), and the Reiter protein complement fixation test (RPCFT) performed manually; the other was a fully automated system using two Technicon AutoAnalyzers (AAII), one for the automated reagin test (AR...

A comparison of the livetin of egg yolk and serum globulin of the common fowl using (a) anaphylactic and (b) precipitin reactions, indicates that the two proteins are very closely related, if not identical. The complement fixation test does not give the same result but indicates a difference between the two proteins. The complement fixation test also shows differences between serum euglobulin a...

The relationship between antibody titres in the microtitration serum agglutination test and the complement fixation test in bovine brucellosis is described. For low and high MSAT values there is good agreement between the 2 tests. This is not the case for MSAT values between 54 and 338 IU/ml. For practical reasons, results falling into this category cannot all be repeated. Repetitions are so st...

After material has been produced for use as an antigen in a complement fixation test it is necessary to find out if the finished product has any complement-fixing ability in the presence of a serum containing a suitable antibody. Moreover, the proposed antigen must not have any undue anticomplementary property, and the " gap" between its anticomplementary titre and its complement fixing titre m...

Interest in the Reiter strain of Treponema pallidum, a nonpathogenic, cultivatable, anaerobic spirochete, has been stimulated by the demonstration that the organism possesses four antigens; one, a thermolabile protein, being group specific for treponemes (D'Alessandro et al., 1949). This antigen has recently been employed in a Reiter protein complement fixation test for syphilis (Cannefax and G...

I N A PREVIOUS PAPER,’ experiments on the application of erythrocyte powder in serologic laboratories were described. It was shown that erythrocyte powder may be successfully used for (1) absorption of useless and disturbing antibodies from test sera, (2) complement fixation test with strong group antibodies, and (3) preparation of “pure” antibodies by elution from the sensitized powder. The ai...