We examined geographic variation within the Ashy Darter, Etheostoma cinereum, of the mitochondrially enconded cytochrome b gene (cyt b) and nuclear recombination activation gene 1 (RAG1) as well as pigmentation, 6 meristic variables, and 20 morphometric variables for patterns indicative of speciation within the complex. Four geographically disjunct entities were identified by at least one of th...