Mycobacterium tuberculosis, a pathogen infecting one third of the world population, faces numerous challenges within the host, including high levels of copper. We have previously shown that M. tuberculosis CsoR is a copper inducible transcriptional regulator. Here we examined the hypothesis that csoR is necessary for maintaining copper homeostasis and surviving under various stress conditions. ...

Copper and iron are essential elements for cellular growth. Although bacteria have to overcome limitations of these metals by affine and selective uptake, excessive amounts of both metals are toxic for the cells. Here we investigated the influences of copper stress on iron homeostasis in Bacillus subtilis, and we present evidence that copper excess leads to imbalances of intracellular iron meta...

Effect of the change of crystallinity and micro texture of electroplated copper thin films by annealing on the stressinduced migration was investigated experimentally and theoretically. The micro texture of electroplated copper thin films changed drastically as a function of their electroplating conditions and the annealing temperature after the electroplating. The crystallinity of the electrop...

Cadmium toxicity interferes with essential metal homeostasis, which is a problem for both plant nutrition and the consumption of healthy food by humans. Copper uptake is performed by the members of the Arabidopsis high affinity copper transporter (COPT) family. One of the members, COPT5, is involved in copper recycling from the vacuole toward the cytosolic compartment. We show herein that copt5...

The size and length effects of an inclusion on multi-pass copper shaped-wire drawing were investigated. For this purpose, an experimental investigation on optimal die half-angle was conducted. Based on experimental data of optimal die half-angle, wire and inclusion deformations, drawing and hydrostatic stress of copper shaped-wires that contain an inclusion were calculated by two-dimensional fi...

background: the early stage of copper poisoning is difficult to be clinically diagnosed in sheep and has not been documented clearly yet. objectives: to assess biomarkers in predicting early copper poisoning in sheep, blood samples were collected from several groups of animals from a naturally copper poisoning occurrence in an industrial region. methods: animals were divided into four experimen...

Including more copper in your everyday diet could be good for your heart, according to a study on page 657. Jiang et al. now find that dietary supplementation of copper offsets the effects of stress on an overworked heart by preventing its enlargement. Copper-carrying proteins disarm oxygen radicals and power electron transport. Humans with copper deficiency have increased cholesterol levels, c...

The gene expression profile of Escherichia coli K-12 MG1655 grown in minimal medium supplemented with elevated copper concentrations (as copper-glycine) has been analysed using whole-genome oligonucleotide microarrays. At 750 muM copper-glycine, the expression of both the cue and cus copper-export systems is evident. At near-lethal copper concentrations (2 mM copper-glycine), the expression of ...

Copper is an essential element in the cellular electron-transport chain, but as a free ion it can catalyze production of damaging radicals. Thus, all life forms attempt to prevent copper toxicity. Plants diminish excess copper in two structural regions: rare hyperaccumulators bind cationic copper to organic ligands in subaerial tissues, whereas widespread metal-tolerant plants segregate copper ...

The advancement of area-array packages, such as flip chips and chip scale packages, has driven the adoption of high density interconnects (HDIs) that allow for an increased number of I/Os with a smaller footprint area. HDI substrates and printed circuit boards use microvias as interconnects between conductor layers. HDIs have evolved from single-level microvias to stacked microvias that travers...