Objective: The application of human cord blood (hCB) is limited to children by using relatively small volume of cord blood that does not contain enough hematopoietic stem cells (HSCs). So, efforts for applying cord blood stem cells in transplantation have led to establishment of some approaches for ex vivo expansion of HSCs such as garcinol. Materials and Methods: CD133+ HSCs were separated by ...

Cord blood hematopoietic stem cells are widely used as an alternative source for hematopoietic stem cells transplant. Increasing rate of patients who need hematopoietic stem cells transplant and many advantages of cord blood in comparison to bone marrow hematopoietic stem cells, have promoted banking of cord blood units. Cord blood banking requires accurate steps in donor selection, cord blood ...

Background The aim of this study was the ex vivo expansion of Umbilical Cord Blood hematopoietic stem cells on biocompatible nanofiber scaffolds. Materials and Methods CD133+ hematopoietic stem cells were separated from umbilical cord blood using MidiMacs (positive selection) system by means of monocolonal antibody CD133 (microbeads) subsequently, flowcytometry method was done to asses...

many investigators have used xenogeneic, especially murine stromal cells and fetal calf serum to maintain and expand human stem cells. the proliferation and expansion of human hematopoietic stem cells in ex vivo culture were examined with the goal of generating a suitable protocol for expanding hematopoietic stem cells for patient transplantation. using primary fetal liver cells, we established...

ex vivo expansion of human umbilical cord blood cells (hucbc) is explored by several investigators to enhance the repopulating potential of hucbc. the proliferation and expansion of human hematopoietic stem cells (hsc) in ex vivo culture was examined with the goal of generating a suitable clinical protocol for expanding hsc for patient transplantation. using primary human mesenchymal stem cells...

abstract background b cells play an important role in the immune system due to production of the immunoglobulin and secreting several cytokines. it has been shown that b cells produced successfully in the presence of stem cell factor (scf) and flt3 ligand (flt3l). il2, il7, and il15 cytokines as  -common family have an essential cytokines may support either synergistically or in an additi...

cord blood hematopoietic stem cells are widely used as an alternative source for hematopoietic stem cells transplant. increasing rate of patients who need hematopoietic stem cells transplant and many advantages of cord blood in comparison to bone marrow hematopoietic stem cells, have promoted banking of cord blood units. cord blood banking requires accurate steps in donor selection, cord blood ...

Abstract Background B cells play an important role in the immune system due to production of the immunoglobulin and secreting several cytokines. It has been shown that B cells produced successfully in the presence of stem cell factor (SCF) and Flt3 ligand (Flt3L). IL2, IL7, and IL15 cytokines as -common family have an essential cytokines may support either synergistically or in an additive m...