PURPOSE In this study, the involvement of signaling pathways in the regulation of HCO(3)(-) permeability across the apical membrane of the corneal endothelium was examined. METHODS Cultured bovine corneal endothelial cells (CBCECs) were grown to confluence on permeable membranes. Apical and basolateral sides were perfused with a HCO(3)(-)-rich Cl(-)-free Ringer's solution (28.5 mM; pH 7.5). R...

In the recent years a significant development of investigations with regard to bioavailability of ocular drugs has been noticed. The corneal epithelial barrier is the main pathway for ocular penetration of topically applied ophthalmic drugs into the anterior chamber. To work out an in vitro model of bovine corneal epithelial primary cultures and exercise it for permeability research with lipoph...

Contact lens extended wear increases the permeability of epithelium to sodium fluorescein (P(dc)). The exact mechanism is not known. However, changes in P(dc) likely result from either corneal hypoxia or mechanical trauma, or both. We explored the effects of one-night continuous wear with either high- or low-Dk/t soft lenses on P(dc). The results show that corneal epithelial barrier function de...

PURPOSE To determine the effects of experimental dryness on production of matrix metalloproteinases (MMPs) and their physiological inhibitors (TIMPs) by the corneal epithelium. METHODS Experimental dry eye (EDE) was created in two strains of mice: BALB/c and C57BL/6. Real-time PCR was performed with MMP and TIMP probes, and the results were analyzed by the comparative C(T) method, selecting t...

With the method of in vitro incubation of the isolated rabbit cornea with the modified KEI medium, the effects of ouabain applied to the endothelial surface have been investigated. The change in corneal hydration with varying concentrations of ouabain was determined; one-half maximum rate of corneal swelling was found at an ouabain concentration of 3 x 10~ moles per liter. Endothelial permeabil...

The endothelial surface of the isolated rabbit cornea was perfused with modified KEI medium, according to the method reported previously. This in vitro cornea maintained its thickness within the normal range even after the epithelium ivas removed. The endothelial permeability of these in vitro corneas to tritiated water, >C-labeled urea, sucrose, and inulin teas determined. The reflection coeff...

• QSPR models were developed based on in vitro corneal permeability measurements of 189 compounds. Partial least squares regression was applied for the model building. Classical molecular descriptors and ECFP fingerprints used. Corneal membrane retention values predicted by models. The capability to predict physicochemical parameters has always been a desirable objective ophthalmic drug develop...

The paracellular permeability of normal rabbit cornea and conjunctiva was studied in vivo and in vitro. After intravenous administration, horseradish peroxidase was found to percolate to the intercellular space of conjunctival epithelia and was restricted by the tight junctions of the superficial epithelium. Only minimal tracer was present in the limbus and cornea. The difference between cornea...

Neovascularization is defined as formation of new vessels from vascular endothelial cells derived from existing blood vessels. These new immature vessels are friable. They have increased permeability, lack structural integrity, and can result in lipid deposition and corneal opacities. Ultrastructurally, corneal neovascularization is characterized by vessels encroaching through separated stromal...