Pentacyanoferrate-bound poly(1-vinylimidazole) (PVI[Fe(CN)5]) was selected as a mediator for amperometric creatinine determination based on the reductive H2O2 detection. Creatinine amidohydrolase (CNH), creatine amidohydrolase (CRH), sarcosine oxidase (SOD), peroxidase (POD), and PVI[Fe(CN)5] were crosslinked with poly(ethylene glycol) diglycidyl ether (PEGDGE) on a glassy carbon (GC) electrode...

The measurement of creatinine in serum is a key indicator of glomerular function of the kidney and its pivotal role in this evaluation has been further increased by recommendations issued by nephrology societies for using equations to estimate the glomerular filtration rate (GFR) [1]. In 2006 the reference system for the measurement of serum creatinine, using suitable higher-order reference mat...

Creatinine is the final product of creatine metabolism in muscle of mammals, and mainly filtered out of blood in kidneys. The creatinine levels are related to the state of renal function, thyroid malfunction and muscular disorders. This indicates the importance of creatinine measurements in blood or urine. The current clinical determination of creatinine is based on Jaffe reaction [1]. However,...

The enzymatic method for the determination of serum creatinine is accepted as one of the standard method in a clinical laboratory. The enzymatic method for the determination of serum creatinine was optimized for use with Merilyzer AutoQuant-400 auto analyzer and its performance characteristics were practically compared with Jaffe’s Kinetic. Effects of some interfering substances like Serum bili...