The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) genome editing system has revolutionized the field of for therapeutic applications genetic diseases and cancers, development disease models, identification targets drug development. However, safe efficient delivery CRISPR/Cas9 in vivo remains a significant challenge. Compared to vira...

Rice blast, caused by the fungus Pyricularia oryzae L., is considered one of main threats to world rice production. The development resistant cultivars best and sustainable control alternatives. Plant breeding efforts have been accelerated genetic mapping (linkage associative) marker assisted selection. On other hand, genomic editing techniques, such as meganucleases (MNs), Zinc-finger nuclease...

Genetic improvement of soybean seed traits is important for developing new varieties that meet the demand as a food, forage crop, and industrial products. A large number genome sequences are currently publicly available. This sequence information provides significant opportunity to design genomic approaches improve traits. Genome editing represents major advancement in biotechnology. The produc...

Abstract The green-flesh (gf) mutant of the tomato fruit ripen to a muddy brown color and has been demonstrated previously be loss-of-function mutant. Here, we provide more evidence support this view that SlSGR1 is involved in change ripening fruits. Knocking out expression using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 genome editing strate...

Fruit and vegetable crops are rich in dietary fibre, vitamins minerals, which vital to human health. However, many biotic stressors (such as pests diseases) abiotic threaten crop growth, quality, yield. Traditional breeding strategies for improving traits include a series of backcrosses selection introduce beneficial into fine germplasm, this process is slow resource-intensive. The new techniqu...

Gene therapy is a potential therapeutic strategy for treating hereditary movement disorders, including hereditary ataxia, dystonia, Huntington's disease, and Parkinson's disease. Genome editing is a type of genetic engineering in which DNA is inserted, deleted or replaced in the genome using modified nucleases. Recently, clustered regularly interspaced short palindromic repeat/CRISPR associated...

The clustered regularly-interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) system from Streptococcus pyogenes (Spy) has been successfully adapted for RNA-guided genome editing in a wide range of organisms. However, numerous reports have indicated that Spy CRISPR-Cas9 systems may have significant off-target cleavage of genomic DNA sequences differing from the intended on-targ...

CRISPR (clustered regularly interspaced short palindromic repeats)-Cas9 (CRISPR-associated protein 9) is a revolutionary tool that utilizes an RNA-guided nuclease for efficient site-directed genome engineering in various eukaryotic systems. The double-strand breaks (DSBs) created by CRISPR-Cas9 are repaired in the cell by two predominant mechanisms: imprecise non-homologous end joining (NHEJ) a...

Prokaryotic organisms survive under constant pressure of viruses. CRISPR-Cas system provides its prokaryotic host with an adaptive immune defense against viruses that have been previously encountered. It consists of two components: Cas-proteins that cleave the foreign DNA and CRISPR array that suits as a virus recognition key. CRISPR array consists of a series of spacers, short pieces of DNA th...