Background: There are varieties of purification techniques for separation of human plasma proteins such as salting out, ion exchange chromatography, and ethanol fractionation. There are limitations for each method, for example in salting out method, the salt has to be removed in an additional step. Ion exchange chromatography is difficult for scaling up, and plasma fractionation is a time consu...

We have isolated and characterized a novel hemolytic protein from the venom of the Hawaiian box jelly®sh (Carybdea alata). Hemolysis of sheep red blood cells was used to quantitate hemolytic potency of crude venom extracted from isolated nematocysts and venom after fractionation and puri®cation procedures. Hemolytic activity of crude venom was reduced or lost after exposure to the proteolytic e...

Isolation and purification of major outer membrane proteins (OMP) from the cell wall envelope of Brucella abortus S-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (Tx-100) and lysozyme treatments, followed by ultracentrifugation. The crude OMP was treated with trypsin to free the preparation from any other protein contaminan...

Because of the general nature of the transamination reaction (1, 2), a study of the kinetics and mechanism of reaction seemed of interest. Preliminary studies of this nature have already appeared (3-5). However, these studies were carried out with crude extracts or resolved enzyme systems. In order to conduct a critical kinetic study as well as a study of the mechanism of reaction, large amount...

isolation and purification of major outer membrane proteins (omp) from the cell wall envelope of brucella abortus s-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (tx-100) and lysozyme treatments, followed by ultracentrifugation. the crude omp was treated with trypsin to free the preparation from any other protein contaminants. t...

Advantages of pelleted feeds in terms of storage, transportation and lower costs compared to natural fresh feed justified the replacement of artificial feeds instead of natural ones partially on maturation performances. This study comprised nine treatments (with three replications) including control treatment: polychaete worm (Perinereis nuntia), Squid and calf liver (natural feed); T1: pellet ...

A polysaccharide depolymerase was puritied 1,688-fold from crude lysates produced by the propagation of a specitic bacteriophage in Pseudomonas aeruginosa. Conventional methods of protein fractionation were employed, namely, salting out with ammonium sulfate, Sephadex gel filtration, and high speed centrifugation. The purified depolymerase behaves as a single entity when tested by electrophores...

A polysaccharide depolymerase was puritied 1,688-fold from crude lysates produced by the propagation of a specitic bacteriophage in Pseudomonas aeruginosa. Conventional methods of protein fractionation were employed, namely, salting out with ammonium sulfate, Sephadex gel filtration, and high speed centrifugation. The purified depolymerase behaves as a single entity when tested by electrophores...