A 45-year-old Japanese man with Sjogren's syndrome developed recurrent skin ulcers, palpable purpura, and dyspnea. Serum mixed-type cryoglobulin level was elevated. A biopsy of his skin lesion showedthe characteristic leukocytoclastic vasculitis of mixed-type cryoglobulinemia. Dyspnea, skin ulcers, and purpura resolved along with a reduction in the serum cryoglobulin level after prednisolone ad...

Studies on the enzymic fragmentation of a single component 6.5 S human cryoglobulin (71: X, Gm factor 4), obtained from a patient suffering from idiopathic cryoglobulinemia, were undertaken to gain a better understanding of the mechanism of temperature-dependent gel formation and its relation to the gross structure of the cryoglobulin molecule. Proteolytic scission of cryoglobulin by papain pro...

blood samples were collected from 20 healthy miniature caspian horses at 37 °c. isolation of cryoglobulin was performed based on a standard method in present study. harvested sera were kept at 4 °c for two hours and then examined for cryoglubolin. four serum samples containing precipitate suspicious of containing cryoglobulin were selected. subsequently serum protein electrophoresis was perform...

Imatinib is a receptor tyrosine kinase inhibitor that blocks the activity of c-Abl, c-Kit, and PDGF receptors. We tested the protective effects of imatinib in thymic stromal lymphopoietin transgenic mice, a model of cryoglobulinemia and associated membranoproliferative glomerulonephritis (MPGN), in which some glomerular manifestations likely result from PDGF receptor activation. Surprising, adm...

Blood samples were collected from 20 healthy miniature Caspian horses at 37 °C. Isolation of cryoglobulin was performed based on a standard method in present study. Harvested sera were kept at 4 °C for two hours and then examined for cryoglubolin. Four serum samples containing precipitate Suspicious of containing cryoglobulin were selected. Subsequently serum protein electrophoresis was perform...

Using two different kinds of monoclonal autoantibodies, anti-mouse RBC (MRBC) autoantibodies and IgG3 rheumatoid factor (RF) cryoglobulins, we have attempted to better define the molecular and cellular basis of the pathogenicity of autoantibodies. Among eight anti-MRBC monoclonal antibodies (mAbs) obtained from NZB mice, only five of them are able to cause anemia. The distinct differences in sp...

E. Smith has pointed out that the use of cryocrit in the investigation of cryoglobulinemia has analytical and clinical limitations, an observation with which we concur in general. The emphasis of our case study, however, was not to make a judgment on which technique should be used, but rather to focus on the importance of including cryoglobulin analysis in the differential and to address the ne...