نتایج جستجو برای: cryopreservation methods

تعداد نتایج: 1880405  

2010
Xia Xu Sally Cowley Christopher J Flaim William James Leonard Seymour Zhanfeng Cui

Human embryonic stem (hES) cells have enormous potential for clinical applications. However, one major challenge is to achieve high cell recovery rate after cryopreservation. Understanding how the conventional cryopreservation protocol fails to protect the cells is a prerequisite for developing efficient and successful cryopreservation methods for hES cell lines and banks. We investigated how t...

Journal: :Human reproduction 2008
V J Onions M R P Mitchell B K Campbell R Webb

BACKGROUND Cryopreservation is hypothesized to result in apoptosis, contributing to stromal damage and follicle loss in ovarian tissue. This study investigated tissue viability following whole ovine ovary cryopreservation and examined the effects of the anti-apoptotic agent sphingosine-1-phosphate (S-1-P) on ovarian cryopreservation efficiency. METHODS Whole ovine ovaries were cryoperfused an...

Journal: :Aesthetic surgery journal 2005
Lee L Q Pu Xiangdong Cui Betsy F Fink Michael L Cibull Dayong Gao

BACKGROUND Several studies have shown that one of the sugars, trehalose, can improve tissue survival after cryopreservation when combined with other cryoprotective agents, and thus may possibly be used in cryopreservation of adipose tissues that have been found more resistant to injury after freezing. OBJECTIVES The purpose of this study was to test our hypothesis that lipoplasty-derived adip...

2009
Shambhu P. Dhital Hira K. Manandhar Hak T. Lim

Cryopreservation has been recognized as a practical and efficient tool for long-term storage of vegetatively propagated plants. This study was conducted to investigate the effects of sucrose concentration, hardening temperature and different cryopreservation methods on the survival rate of potato shoot tips after cryopreservation. Excised shoot tips of in vitro plantlets of potato cultivars, At...

Journal: :Cancer treatment and research 2010
Michelle L McGowan

The goals and rhetoric of The Oncofertility Consortium [1] are aimed toward preserving cancer patients’ reproductive choices and facilitating their reproductive autonomy after cancer. While the end goals of oncofertility research are oriented toward safeguarding the possibility of biological reproduction for women and girls facing cancer treatments that may affect their reproductive capacity, c...

2013
Éva Váradi Barbara Végi Krisztina Liptói Judit Barna

Conservation of indigenous poultry species is an important part of the new Hungarian agricultural strategy. Semen cryopreservation is the most practical method for the long term storage of poultry genetic material. The objective was to compare four protocols for cryopreservation of guinea fowl sperm (slow and fast programmable, freezing in nitrogen vapor, and pellet) and three cryoprotectants (...

2012
I. Demeestere O. Basso F. Moffa F. Peccatori C. Poirot E. Shalom-Paz

With improved survival rates among cancer patients, fertility preservation is now being recognized as an issue of great importance. There are currently several methods of fertility preservation available in female cancer patients and the options and techniques via assisted reproduction and cryopreservation are increasing, but some are still experimental and continues to be evaluated. The establ...

Journal: :Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis 2006
K K Fleming A Hubel

Recent studies illustrate the potential for improving the cryopreservation of stem cells. Reduced DMSO concentrations in the cryopreservation medium, post thaw washing of cells and increased cell concentration have been actively studied. Standardization of cell processing has led to the study of liquid storage prior to cryopreservation, validation of mechanical (uncontrolled rate freezing) free...

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