Non-human primates (NHPs) are confirmed as reservoirs of Cryptosporidium spp., Giardia intestinalis, and Enterocytozoon bieneusi. In this study, 197 fresh fecal samples from 8 NHP species in Qinling Mountains, northwestern China, were collected and examined using multilocus sequence typing (MLST) method. The results showed that 35 (17.8%) samples were positive for tested parasites, including Cr...

objective :cryptosporidiosis is one of the most important parasitic infections in iran which causes diarrhea in humans and animals. the identification of the cryptosporidium species among humans is necessary. this study aims to identify species of cryptosporidium isolated from patients that referred to three hospitals in tehran based on the 18s rrna gene by nested pcr-rflp assay. ‎methods :in t...

We analyzed 1,042 Cryptosporidium oocyst-positive slides (456 from raw waters and 586 from drinking waters) of which 55.7% contained 1 or 2 oocysts, to determine species/genotypes present in Scottish waters. Two nested PCR-restriction fragment length polymorphism (RFLP) assays targeting different loci (1 and 2) of the hypervariable region of the 18S rRNA gene were used for species identificatio...

Four lambs 4 months old were inoculated with Cryptosporidium andersoni oocysts (bovine isolate) in a dose of 5 x 10(6) per animal followed later by 1 x 10(7) oocysts per animal. No animal shed Cryptosporidium oocysts during 56 days post infection, no lamb showed clinical symptoms of cryptosporidiosis and no macroscopic changes were detected in the abomasum immediately after autopsy. Histologica...

the protozoan intestinal parasite cryptosporidium commonly infects cattle throughout the world and iran. the present study was undertaken to determine the abundance and associated risk factors of cryptosporidium infection in cattle herds of northwestern iran. a total number of 246 fecal samples from 138 (56.1%) diarrheic (d) and 108 (43.9%) non-diarrheic (nd) cattle were randomly collected and ...

Nucleotide sequences of the Cryptosporidium oocyst wall protein (COWP) gene were obtained from various Cryptosporidium spp. (C. wrairi, C. felis, C. meleagridis, C. baileyi, C. andersoni, C. muris, and C. serpentis) and C. parvum genotypes (human, bovine, monkey, marsupial, ferret, mouse, pig, and dog). Significant diversity was observed among species and genotypes in the primer and target regi...

In this study, we identified Cryptosporidium species and genotypes present in dairy cattle in the central region of São Paulo state, Brazil. Fecal specimens were collected from 200 animals (100 calves and 100 cows) in ten dairy farms. Fecal samples were examined using microscopic examination (ME), enzyme immunoassay (EIA) and polymerase chain reaction (PCR). Cryptosporidium species and genotype...

The protozoan intestinal parasite Cryptosporidium commonly infects cattle throughout the world and Iran. The present study was undertaken to determine the abundance and associated risk factors of Cryptosporidium infection in cattle herds of northwestern Iran. A total number of 246 fecal samples from 138 (56.1%) diarrheic (D) and 108 (43.9%) non-diarrheic (ND) cattle were rando...

A total of 333 fecal specimens from horses in southwestern China were genotyped based on analysis of the small subunit rRNA (SSU rRNA) gene. Cryptosporidium hominis and Cryptosporidium andersoni were identified in 2 and 4 stool specimens, respectively. The identification of C. hominis was confirmed by sequence analysis of the 70-kDa heat shock protein (HSP70) and oocyst wall protein (COWP) gene...

Giardia and Cryptosporidium are important causes of diarrhoea in Bangladesh. The high prevalence of both parasites in humans and cattle in rural Bangladesh and the common use of water ponds by village inhabitants and their animals suggest a potential for zoonotic transmission. Direct transmission of Giardia and Cryptosporidium between cattle and their handlers and indirect transmission through ...