The arginine-binding protein from Thermotoga maritima (TmArgBP) is an arginine-binding component of the ATP-binding cassette (ABC) transport system in this hyperthermophilic bacterium. This protein is endowed with an extraordinary stability towards thermal and chemical denaturation. Its structural characterization may provide useful insights for the clarification of structure-stability relation...

The purification and crystallization of two different crystal forms of the two-domain protein bovine cyclophilin 40 is reported. Tetragonal crystals grown in methyl pentanediol belong to space group P4222 with unit-cell parameters a = 94.5, c = 118.3 A. Long thin needles grown from PEG belong to space group C2 with unit-cell parameters a = 125.71, b = 47.3, c = 74.6 A, beta = 93.90 degrees. The...

Mouse monoclonal IgE anti-2,6-dinitrophenyl antibody (clone SPE-7) crystallizes in two dimensions when bound to a phospholipid monolayer or bilayer that has been combined with the hapten 2,6-dinitrophenyl groups. The two-dimensional lattice exhibits considerable local disorder, but long-range order is maintained over many unit cells of the lattice. The crystallization of IgE on the films is sen...

Two versions of the functional core of the rabbit voltage-dependent calcium channel beta2a subunit were expressed in Escherichia coli. These proteins were purified to homogeneity and screened for crystallization. Crystallization conditions were refined using the hanging-drop vapour-diffusion method and two crystal forms were pursued. Crystal form I is represented by thick rods with tetragonal s...

The title compound, C(12)H(16)N(4)O(3)S·H(2)O, which has potential as a possible anti-malarial drug, was studied when small deviations in melting points, for two differently aged preparations, were observed. The unexpected existence of a water mol-ecule of crystallization is considered to be the cause of this variation. The 7H-pyrrolo-[2,3-d]pyrimidine unit is very slightly puckered with a tota...

The production of diffraction-quality crystals of Munc18c, a protein involved in regulating vesicular exocytosis in mammals, is reported. The diffraction resolution of Munc18c crystals was optimized by (i) cocrystallizing with a peptide fragment of the Munc18c functional binding partner syntaxin4, (ii) using nanolitre free-interface diffusion crystallization-screening chips and microlitre hangi...

Although protein crystallization is a unit operation with potentially high separation factors, it has not been widely used in industry. Protein crystallization studies and practices have hitherto been largely limited to crystallography protocols. Knowledge of the behaviour of protein in solution would help to overcome empiric limitations in protein crystallisation. Thus, dissolution of porcine ...

The maximum number of non-overlapping unit spheres in R that can simultaneously touch another unit sphere is given by the kissing number, k(3) = 12. Here, we present a proof that the maximum number of tangencies in any kissing configuration is 24 and that, up to isomorphism, there are only two configurations for which this maximum is achieved. The result is motivated by a three-dimensional crys...

Crystallization is a key unit operation that finds extensive applications in pharmaceutical, food and fine chemicals industries . The operating conditions of the crystallization process have a direct effect on the purity, size distribution, shape and polymorphic form of the crystals. These properties in turn affect the efficiencies of the downstream operations such as filtration, drying and for...

In this paper, the purification, crystallization and preliminary X-ray crystallographic studies of human carnitine acetyltransferase are reported. Recombinant human carnitine acetyltransferase crystals were grown by the hanging-drop vapor-diffusion method and belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 137.65, b = 84.76, c = 57.65 A and one molecule per a...