Protoplasts isolated from tobacco leaves were inoculated with cucumber mosaic virus (CMV) using a method slightly modified from that developed for inoculating tobacco mosaic virus. Multiplication of CMV was demonstrated by staining the protoplasm with fluorescent CMV-antibody, by the electron microscopy of ultrathin sections, and by assaying the infectivity in protoplasts. Seventy to 90% of pro...

S 5 CHAPTER I: Deletion of the C-Terminal 33 Amino Acids of Cucumber Mosaic 7 Virus Movement Protein Enables a Chimeric Brome Mosaic Virus to Move from Cell

Interstrain recombinants were observed in the progenies of the Cucumber mosaic virus (CMV) reassortant L(1)L(2)F(3) containing RNAs 1 and 2 from LS-CMV and RNA 3 from Fny-CMV. We characterized these recombinants, and we found that their fixation was controlled by the nature of the replicating RNAs 1 and 2. We demonstrate that the 2b gene partially affects this fixation process, but only in the ...

Pepper lines L57 and L113 and cv.Pico are inoculated by 1, 5, 10, 25, 75 μg/ml cucumber mosaic virus (CMV) in inoculum. Both lines are resistant to the virus and cv.Pico is used as a susceptible control. The lines respond with the development of chlorotic (L57) and necrotic (L113) spots on the inoculated leaf. The virus is established only in the inoculated leaves and their petioles of the resi...

A substantial relationship between cucumber viruses 3 and 4 and certain members of the tobacco mosaic virus group may be demonstrated by a comparison of their physicochemical properties and by serological tests (l-3). However, the test which would establish conclusively kinship between these nucleoproteins, namely the immunization of a plant diseased with cucumber virus 3 or 4 against infection...

Much work has been done on the isolation, purification, and characterization of the RNA-directed RNA polymerase (EC 2.7.7.48) of cucumber mosaic virus (CMV)-infected cucumbers. Uninfected plants were reported to have no such enzyme, but we recently detected low levels of the activity in cucumber. Since tobacco and cowpea contain such an enzyme that is variably increased in amount by various vir...

Cucumber plants exhibiting virus-like disease symptoms were collected in various gardens and greenhouses in Lithuania. Viral diseases were expressed by symptoms of mosaicmottling, yellow ringspots and vein necrosis of leaves. Growth and fruiting of plants were reduced. Results of the identification of viruses detected in cucumbers by the RT-PCR technique are presented here. Identification of ca...