The most physiological type of cell cycle arrest - namely, contact inhibition in dense culture - is the least densely studied. Despite cell cycle arrest, confluent cells do not become senescent. We recently described that mTOR (target of rapamycin) is inactive in contact-inhibited cells. Therefore, conversion from reversible arrest to senescence (geroconversion) is suppressed. I this Perspectiv...

Primary mouse embryo cells, when placed on a regular pattern of replating, initially exhibit a cyclical pattern of proliferation. When such cells recover from the primary injury caused by the carcinogen methylnitrosourea, they acquire a markedly enhanced plating efficiency. This change is independent of the density of plating at which the cells were treated and is probably a true, induced, heri...

A number of lines of rabbit lens epithelium have been groion in large-scale continuous tissue culture for periods of 1 to 2 years. The cells exhibit a marked degree of contact inhibition on the monolayer, suggesting that this phenomenon may play a role in the regulation of cell division in vivo. The availability of large numbers of homogeneous cells has permitted determination of the life cycle...

The identification of fluorescently stained cell nuclei is the basis of cell detection, segmentation, and feature extraction in high content microscopy experiments. The nuclear morphology of single cells is also one of the essential indicators of phenotypic variation. However, the cells used in experiments can lose their contact inhibition, and can therefore pile up on top of each other, making...

Acanthamoeba keratitis is an unusual complication of wearing soft contact lenses: a case was diagnosed by culturing corneal scrapings. Acanthamoeba are tolerant of variations in osmolarity in the culture media. Page's saline, recommended for culture agar plates, can easily be substituted with salines which are suitable for cell culture media, thereby avoiding the cumbersome manufacture of speci...

background: amoebic keratitis introduced as a painful corneal infection which sometimes lead to poor vision and blind-ness. the main goal of this study was to report amoebic keratitis during ten years from 1997-2007 in patients who was sus-pected to have amoebic keratitis and referred to parasitology laboratory, school of public health, tehran univer¬sity of medical sciences, iran. other aim wa...

BACKGROUND There are conflicting results regarding the role of periendothelial mural cells in angiogenesis. In the current study, we investigated the role of mesangial cells (MCs) in endothelial vascularization by using a three-dimensional co-culture system in basement-membrane reconstruct gel (Matrigel). METHODS Human umbilical vein endothelial cells (ECs) and human MCs were used. In the con...