Monoclonal antibody producing Chinese hamster ovary (CHO) cells have been shown to undergo metabolic changes when engineered to produce high titers of recombinant proteins. In this work, we have studied the distinct metabolism of CHO cell clones harboring an efficient inducible expression system, based on the cumate gene switch, and displaying different expression levels, high and low productiv...

Pseudomonas putida F1 can assimilate benzene, toluene and ethylbenzene using the toluene degradation pathway, and can also utilize p-cymene via p-cumate using the p-cymene and p-cumate catabolic pathways. In the present study, P. putida F1 strains were isolated that were adapted to assimilate new substrates such as n-propylbenzene, n-butylbenzene, cumene and biphenyl, and the molecular mechanis...

Altimetry has become the focus of global climate studies because it is the only tool available able to provide both mesoscale (100-500 kIn) and basin-scale (10,000 kIn) circulation information on a global, synoptic basis. . The Atlas-launched Navy Geosat mission proved the capability of the radar altimeter to measure the mesoscale ocean features. A lightsat compatible Geosat Follow-On Technolog...

BACKGROUND Methylotrophic (methanol-utilizing) bacteria offer great potential as cell factories in the production of numerous products from biomass-derived methanol. Bio-methanol is essentially a non-food substrate, an advantage over sugar-utilizing cell factories. Low-value products as well as fine chemicals and advanced materials are envisageable from methanol. For example, several methylotro...

11 Tunable promoters represent a pivotal genetic tool for a wide range of applications. 12 Here we present such a system for sphingomonads, a phylogenetically diverse group of 13 bacteria that have gained much interest for their potential in bioremediation and their 14 use in industry and for which no dedicated inducible gene expression system has been 15 described so far. A strong, constitutiv...

PmxaF is a strong methanol-inducible promoter in Methylobacterium extorquens. When this promoter is cloned in expression vectors and used to drive heterologous gene expression, methanol inducibility is either greatly reduced or entirely lost. In order to bestow inducibility upon the cloned PmxaF promoter in expression vectors, we adopted combinational methods (regulatory elements of the Pseudom...