to evaluate the effects of calcium on motility patterns of bovine epididymal sperm and to determine the effective calcium concentration, ovine testicles (5 for each experiment day) were picked up in a local slaughterhouse and transported to the laboratory in a cool container (filled wich ice).in laboratory,caudal epididymis was incised and sperm cells were put into petri dishes containing modif...

The objective of this study was to evaluate the quality of thawed semen of Holstein bull by computer assisted sperm analysis (CASA). A total sample of 40 straws imported from USA (total bulls = 4; 5 ejaculates per bull; 2 straws per ejaculate) was analysed by CASA using an IVOS. Semen motility was evaluated for kinetics parameters after thawing. The percentage of total mobile spermatozoa (%TMOB...

Background: Optimal thawing rate is critical for quality and fertilizability of frozen -thawed spermatozoa. Kinetic parameters are the most important factors which are affected by thawing rate so, that have close relation between kinetic parameters and fertility of the spermatozoa. Tris - egg yolk have been widely used for cryopreservation of the buffalo and other species semen. According to ou...

The purpose of this study is to analyze human sperm motility and energetics in media with different viscosities. Multiple experiments were performed to collect motility parameters using customized computer tracking software that measures the curvilinear velocity (VCL) and the minimum laser power (Pesc) necessary to hold an individual sperm in an optical trap. The Pesc was measured by using a 10...

Motility is an essential characteristic of all flagellated spermatozoa and assessment of this parameter is one criterion for most semen or sperm evaluations. Computer-aided sperm analysis (CASA) can be used to measure sperm motility more objectively and accurately than manual methods, provided that analysis techniques are standardized. Previous studies have shown that evaluation of sperm subpop...

Measurement of sperm velocity parameters takes an important place in analysis. Today, computer-assisted analysis (CASA) systems are used to detect motility measurements such as VCL (Curvilinear Velocity), VSL (Linear Velocity) and VAP (Average Path Velocity). The path length cells is necessary calculate the it calculated using video processing. However, this obtained by discrete-time processing...

the aim of this study was to evaluate the effects of dilution rate and holding time on various motility parameters using computer-assisted sperm analysis (casa). the semen samples were collected from three ghezel rams. samples were diluted in seminal plasma (sp), phosphate-buffered saline (pbs) containing 1% bovine serum albumin (bsa) and bioexcell. the motility parameters that computed and rec...

The effects of sauna exposure on sperm movement characteristics and other semen parameters were evaluated using computer-assisted sperm analysis (CASA). A significant (p < 0.01) decrease in average path velocity (VAP), curvilinear velocity (VCL) and amplitude of lateral head displacement (ALH) was found after exposure to sauna for 2 weeks. The altered parameters returned to their original value...

The Aim: aim of the study was to investigate applicability an up-to-date method, sperm selection with nanoparticles at different temperatures (37ºC, 21ºC, and 4ºC), on Angora buck semen. Material Methods: Semen samples were collected from 3 bucks 7 repetitions each sample diluted divided into 4 groups. Nanopurification procedure applied by using a nanoparticle dose 10 μg/ml three groups 37ºC, 4...

Background: There is a general deterioration in sperm quality depending on time postmortem. Refrigeration of the epididymes down to around 5°C is the best strategy to lower this damage and there are many dissim ilarities between species, possibly due to differences on cold shock endurance of epididymal sperm. Our objective was to determine the effect of 5°C and 37°C on goat epididymal sperms mo...