Stimulated by its success in both bioconjugation and surface modification, we studied the strain-promoted oxidation-controlled cycloalkyne-1,2-quinone cycloaddition (SPOCQ) in three ways. First, the second-order rate constants and activation parameters (ΔH⧧) were determined of various cyclooctynes reacting with 4-tert-butyl-1,2-quinone in a SPOCQ reaction, yielding values for ΔH⧧ of 4.5, 7.3, a...

Genetic code expansion is a useful method to study metalloproteins and protein posttranslational modifications. We constructed several tyrosine and pyrrolysine tRNA synthetase libraries based on tyrosine and pyrrolysine tRNA synthetase/tRNA pairs to screen unnatural amino acids (UAAs) with similar chemical structure to tyrosine or lysine. We had incorporated several unnatural amino acids into p...

In this study, reactions of the simple cycloalkynes with substituted Nitrile Oxides, by DFT method will be discussed. The investigation of the structural properties, theoretical thermodynamic and kinetic data, i.e., the activation free energies(DG*), the free energies changes of reaction(DrG) and rate constants of the reactions (k) in 298 K and effects of Electron-withdrawing and...

The photoelectron spectrum of the dehydrocyclooctatetraene negative ion, C8H6, is reported. The spectrum strongly resembles that previously reported for the cyclooctatetraene anion, indicating that the structure of C8H6 is very similar to that of C8H8. Two electronic states of dehydrocyclooctatetraene are observed in the photoelectron spectrum. The lowest energy feature is assigned to singlet 1...