Since bacterial cells are basically similar to other living cells (Mudd, 1954; DeLamater, 1954) they are also susceptible to the histochemical analyses performed on higher cells. The present study, employing histochemical techniques, may help to resolve some of the problems and areas of disagreement posed by previous investigations in bacterial cytology (Bisset, 1951; Robinow and Hannay, 1953; ...

This review is intended to give a general idea of the basic methods for studying structure cells and bacteria. direction great importance any clinician gaining immense popularity around world due its accuracy cost-effectiveness. considers such areas as cytomorphology, cytophysiology, cytochemistry cytogenetics.

Enzyme cytochemistry has been used, at the light and electron microscope levels, to "mark" cytoplasmic organelles of mammalian cells. Catalase cytochemistry permitted identification of microperoxisomes, apparently ubiquitous organelles that are attached by numerous slender connections to the endoplasmic reticulum. Thiamine pyrophosphatase and acid phosphatase cytochemistry can be used to distin...

Acid phosphatase localization in accumulated membranous organelles of optic nerve axons of guinea pigs following acute elevation of intraocular pressure (IOP) was determined, employing light and electron microscopic enzymic cytochemistry with beta-glycerophosphate as a substrate. Positive reaction products appeared to accumulate in the region of the lamina cribrosa, as revealed with light micro...

For many pathologists, only pedantry can resolve the difference between the terms "histochemistry" and "cytochemistry". Moreover, the better known subject of histochemistry is not distinguished for its quantification. So it may come as some surprise, that the most sensitive bioassays, of polypeptide hormones, that are currently available and that are about one thousand times as sensitive as the...

Freeze-fracture cytochemistry allows visualization of cellular and molecular characteristics of biomembranes in situ. In this review, we discuss freeze-fracture cytochemistry with special reference to a new cytochemical labeling of replicas, the detergent-digestion fracture-labeling technique. In this procedure, unfixed cells are rapidly-frozen, freeze-fractured, and physically stabilized by ev...