نتایج جستجو برای: cytopathic effect cpe

تعداد نتایج: 1644232  

2016
John Martin

Submit Manuscript | http://medcraveonline.com Abbreviations: ACE: Alternative Cellular Energy; HIV: Human Immunodeficiency Virus; HSV: Herpes Simplex Virus; HZV: Herpes Zoster Virus; HPV: Human Papillomaviruses; KELEA: Kinetic Energy Limiting Electrostatic Attraction; SCMV: African Green Monkey Simian Cytomegalovirus; CPE: Cytopathic Effect; CTL: Cytotoxic T Lymphocytes; CAM: Complementary and ...

2011
Jie Li Dong-mei Hu Xi-xia Ding Yue Chen Yu-xian Pan Li-wen Qiu Xiao-yan Che

A dengue nonstructural protein 1 (NS1) antigen capture enzyme-linked immunosorbent assay (ELISA)-based tissue culture infectious dose-50 (TCID(50)) test (TCID(50)-ELISA) was developed as an alternative to the standard plaque assay for titrating dengue virus. Virus titers obtained by TCID(50)-ELISA were comparable to those obtained by the plaque assay and by the traditional TCID(50)-cytopathic e...

Journal: :Applied microbiology 1970
C T Cho T Locke H A Wenner

Rabbit pox virus (RPV) produced cytopathic effect (CPE) in five types of cells grown in tissue cultures. The CPE on CV-1 cells was characterized by cell fusion and lysis. The CV-1 line is a useful and sensitive cell culture for measuring concentrations of RPV in blood and tissues of infected rabbits. Viremia was detected between the 2nd and 4th days after parenteral infection. By the 6th and 7t...

Journal: :Journal of virological methods 2006
Oliver Schildgen Maarten F Jebbink Michel de Vries Krzysztov Pyrc Ronald Dijkman Arne Simon Andreas Müller Bernd Kupfer Lia van der Hoek

Six cell lines routinely used in laboratories were tested for permissiveness to the infection with the newly identified human coronavirus NL63. Two monkey epithelial cell lines, LLC-MK2 and Vero-B4, showed a cytopathic effect (CPE) and clear viral replication, whereas no CPE or replication was observed in human lung fibroblasts MRC-5s. In Rhabdomyosarcoma cells, Madin-Darby-Canine-kidney cells ...

Journal: :Journal of virology 2006
Natalia Garmashova Rodion Gorchakov Elena Frolova Ilya Frolov

Replication of alphaviruses in vertebrate cells strongly affects cell physiology and ultimately leads to development of a cytopathic effect (CPE) and cell death. Sindbis virus (SIN) replication causes major changes in cellular macromolecular synthesis, in which the strong downregulation of transcription of cellular mRNAs and rRNAs plays a critical role. SIN nonstructural protein nsP2 was previo...

Journal: :Journal of clinical microbiology 1986
M Q Arens E M Swierkosz R R Schmidt T Armstrong K A Rivetna

During two winter seasons, we found that the combination of WI-38 or MRC-5 human lung fibroblasts plus primary rhesus monkey kidney (RhMK) and HEp-2 cell cultures yielded maximal isolation of respiratory syncytial virus. Cytopathic effects (CPE) developed earliest in RhMK cells and slowest in the human fibroblast lines. In RhMK cells, 50% of ultimately positive cultures showed CPE in 5 days, an...

2012
Di Tian Wanju Zhang Jing He Yi Liu Zhigang Song Zhitong Zhou Min Zheng Yunwen Hu

A novel electronic cell sensor array technology, the real-time cell analysis (RTCA) system, was developed to monitor cell events. Unlike the conventional methods labeling the target cells with fluorescence, luminescence, or light absorption, the RTCA system allows for label-free detection of cell processes directly without the incorporation of labels. Here, we used this new format to measure th...

Journal: :The Malaysian journal of pathology 1990
D Sabil S K Othman I Isahak

150 specimens from suspected herpes simplex genital and skin lesions were received in virus transport medium. They were inoculated into Hep-2 cell monolayers, examined for the presence of virus by cytopathic effect (CPE), direct immunoperoxidase (DIP) and direct immunofluorescence (DIF). Of 39 (26.0%) virus-positive specimens by CPE, 37 (24.7%) were HSV-positive by DIP and 36 (24.0%) by DIF sta...

Journal: :Journal of clinical microbiology 2000
N A Khan E L Jarroll N Panjwani Z Cao T A Paget

Acanthamoeba keratitis is a vision-threatening infection caused by pathogenic species of the genus Acanthamoeba. Although not all Acanthamoeba spp. can cause keratitis, it is important to differentiate pathogenic species and isolates from nonpathogens. Since extracellular proteases may play a role in ocular pathology, we used colorimetric, cytopathic, and zymographic assays to assess extracellu...

Journal: :Journal of virology 2005
Olga Petrakova Eugenia Volkova Rodion Gorchakov Slobodan Paessler Richard M Kinney Ilya Frolov

Venezuelan equine encephalitis (VEE) and eastern equine encephalitis (EEE) viruses are important, naturally emerging zoonotic viruses. They are significant human and equine pathogens which still pose a serious public health threat. Both VEE and EEE cause chronic infection in mosquitoes and persistent or chronic infection in mosquito-derived cell lines. In contrast, vertebrate hosts infected wit...

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