The cytotoxicity of biomateriats can be tested in vitro using various culture systems. Liquid culture systems may detect cytotoxicity of a material either by culture of cells with extracts or with the material itself. In the latter instance, renewing the medium will remove possible released cytotoxic products. The agar-overlay test is a short term semi-solid culture system in which the possible...

introduction: this study aimed to compare the cytotoxicity of mta fillapex, ah-26 and apatite root canal sealers at different times after mixing. methods and materials: in this in vitro study, mta fillapex, ah-26 and apatite root canal sealer were spilled uniformly by 40 µm mesh in a 96-well plate. then, human fetal foreskin fibroblast cell line (hfff2) were added to each sealer cell culture me...

introduction: the aim of this study was to compare the cytotoxicity of proroot mta (pmta), root mta (rmta) and portland cement (pc) on human gingival fibroblasts (hgfs). materials and methods: the extracts of the test materials were placed close to human gingival fibroblasts (hgfs) within 96-well plates. cellular viability was assessed using mtt assay in different intervals (freshly mixed, 4, 2...

The purpose of this study was to evaluate and compare the cytotoxicity and gene expression profiles in cell cultures exposed to whole smoke generated from a full flavor cigarette (Test 1), a low tar cigarette (Test 2), and an ultra-low tar cigarette (Test 3). In addition, a reference cigarette 2R4F was evaluated for cytotoxicity. Neutral red (NR) cytotoxicity assay was performed to determine re...