Fifty three alkaloids were identified in the organs (roots, stems, leaves, flowers, and seeds) of Datura innoxia by GC/MS. Seventeen of them are reported for the first time for this species and one nor-derivative, 3-phenylacetoxynortropane (28), for the genus Datura. Furthermore, four new tropane esters were tentatively identified as 3-acetoxy-6,7-epoxytropane (acetylscopine) (10), 3-acetoxy-6-...

For the determination of the hyoscyamine-scopolamine content in small quantities of fresh plant tissue an HPLC assay was developed; with a sensitivity of about 20 ng alkaloid per run. Analysis of the different organs of Datura innoxia plants are in accord with the concept that hyoscyamine is synthesized in the roots, whereas transformation to scopolamine starts in these organs and proceeds in t...

Using haploid and diploid Datura innoxia Mill, callus cultures and cell suspensions it was shown that meristematic material usually attains the lowest possible C-value of a given ploidy level. Parenchyma material of callus cultures, however, indicates a broad scattering of C-values up to 16 C, which is paralleled by C-value distribution of the free cell fraction in actively dividing cell suspen...

Tropane alkaloids present a great economic value in particular for pharmaceutical industry. The culture of Datura sp hairy roots obtained by inoculation with A4 Agrobacterium rhizogenes offers promising prospects for alkaloids in-vitro production. The objective of our study is to try optimizing hyoscyamine production exploiting the composition of mineral fraction of the culture media. On the wh...

The kinetics of tropane alkaloids accumulation in different organs such as roots, leaves, stems, flowers and seeds of Datura innoxia was investigated by GC-MS. Twenty-six tropane alkaloids were detected. The ester derivatives of tropine (3alpha-tigloyloxytropine and 3-tigloyloxy-6-hydroxytropine) are the major compounds. Undifferentiated callus were established from the stem explants of Datura ...

Datura innoxia cells from suspension cultures were selected for their ability to grow and divide rapidly in normally lethal concentrations of cadmium. Cells resistant to 12.5, 25, 50, 100, 160, 200, and 250 micromolar cadmium chloride were isolated and utilized to initiate cell suspension cultures resistant to this toxic metal ion. Variant cell lines retained their ability to grow in cadmium af...

Noteworthy records of 11 alien plant species in Kyiv City and Region made mainly 2020 are discussed. In particular, new localities the following taxa reported: Amaranthus spinosus, Artemisia tournefortiana, Chenopodium ucrainicum, Celastrus orbiculatus, Datura innoxia, Dysphania ambrosioides, Erechtites hieraciifolius, Mesembryanthemum ×vascosilvae (M. cordifolium × M. haeckelianum; recently de...

The uptake and biotransformation of TNT was studied in cell suspension cultures and in whole plants of Datura innoxia and Lycopersicon peruvianum. In cell culture, TNT was rapidly removed from the growth medium and recovered from the cell extract in the form of a variety of biotransformation products resulting from nitroreduction, deamination, Nacetylation and side chain oxidation to aldehyde a...

The cytokinin complex of Datura innoxia Mill. crown gall tissue was purified by ion exchange, Sephadex LH-20 chromatography and reversed-phase high performance liquid chromatography. By gas chromatography-mass spectrometry using (2)H-labeled compounds, the following cytokinins were identified in the basic fraction eluting from a cation exchange column: zeatin, zeatin riboside, dihydrozeatin, di...