The effect of diabetes in vivo has not been examined on isolated podocytes. To achieve this, GFP was expressed constitutively in podocytes of PGFP transgenic mice which were bred to OVE mice to produce diabetic OVE-GFP mice. Viewing GFP fluorescence, foot processes of OVE-GFP podocytes were visually and measurably effaced, which did not occur with less severe STZ diabetes. Over 300,000 podocyte...

Continuous-flow mass spectrometry (CFMS) was used to monitor the products formed during the initial 0.25-20 s of the reactions catalyzed by the flavoprotein N-acetylpolyamine oxidase (PAO) and the pterin-dependent enzymes phenylalanine hydroxylase (PheH) and tyrosine hydroxylase (TyrH). N,N'-Dibenzyl-1,4-diaminobutane (DBDB) is a substrate for PAO for which amine oxidation is rate-limiting. CFM...

Abstract Introduction Increased reactive oxygen species are often correlated with erectile dysfunction (ED) by enhancing contractility and favoring the flaccid state of penis (which is mainly mediated α-adrenergic innervation) decreasing relaxation corpus cavernosum (CC) associated decreased nitric oxide (NO) bioavailability. Leucine Rich Repeat Containing 8 (LRRC8) Volume Regulated Anion Chann...

The interaction of water vapour with Al-MCM-41, prepared by direct synthesis at ambient temperature and pressure, using tetraethoxysilane, aluminium sulfate, hexadecyltrimethylammonium bromide and ammonia, and its effect on the pore structure were studied in order to investigate the stability towards prolonged exposure to water vapour and the influence of the aluminium content. With this purpos...

Demam Berdarah Dengue (DBD) merupakan suatu penyakit yang disebabkan oleh infeksi virus dengue. DBD salah satu dari empat serotipe genus flavivirus. Penyakit ini berbahaya, sehingga perlu dilakukan prediksi untuk mengatahui wilayah mana saja rawan terkena kasus DBD. Melakukan tersebut bertujuan agar dapat dengan segera mengetahui tingkat penyebaranya tinggi hingga rendah melakukan tindakan dan ...

We present a database of structured, verified and useful information about cysteine residues involved in disulfide bridges. The database has been filled with proteins of a subset of the PDB database called select 25. A rough analysis of the proteins contained in the database shows that it is possible to identify cysteine residues which are involved or not in a disulfide bond from the amino acid...