Genetic manipulation of organisms using CRISPR/Cas9 technology generally produces small insertions/deletions (indels) that can be difficult to detect. Here, we describe a technique to easily and rapidly identify such indels. Sequence-identified mutations that alter a restriction enzyme recognition site can be readily distinguished from wild-type alleles using a cleaved amplified polymorphic seq...

to study the molecular basis for resistance of lolium rigidum populations (from fars province) to aryloxyphenoxy propionate herbicide through dcaps method, an experiment was conducted at the biotechnology laboratory of shiraz university. eighteen populations of l. rigium suspected of herbicide resistance, from zarghan, fasa. noorabad and firoozabad areas which had survived a recomended dose of ...

We report AtPRIMER, an application that automates the discovery of new polymorphic markers between ecotypes of Arabidopsis thaliana. On specifying two ecotypes and the genomic region of interest, the script retrieves all corresponding single nucleotide polymorphisms (SNPs) and generates CAPS and/or dCAPS PCR primer sequences. We show that AtPRIMER accurately found specific polymorphic markers f...

Abstract The simultaneous pursuit of success in elite sport and the highest school qualification is considered to be one challenges for adolescents who are sporting talents. Dual career assistance programmes (DCAPs) student-athletes designed help master this challenge best possible way. This study focusses on upper secondary performance 200 from an sports school. First, enrolled two different D...

Cadmium (Cd) is a pollutant metal present in soils and toxic to biologic organisms. Previous studies using recombinant inbred lines derived from a cross between soybean (Glycine max [L.] Merr.) cultivars Harosoy and Fukuyutaka revealed a major quantitative trait loci for seed Cd concentration (cd1) in chromosome 9. The genome sequence of Williams 82 suggested that a P(1B)-ATPase gene involved i...

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