نتایج جستجو برای: decellularization

تعداد نتایج: 565  

Introduction: Tissue engineering by using decellularized tissues has been attracted attention of researchers in the regenerative medicine. Extra cellular matrix (ECM) is a secretory product of cells inside the tissues with supportive and regulatory function for homing cells. ECM contains glycosaminoglycans (GAGs) and fibrous proteins. Each particular tissue has its unique ECM, especially brain,...

Journal: :Tissue engineering. Part A 2013
Hong-Gook Lim Sun Young Choi Eun Jung Yoon Soo Hwan Kim Yong Jin Kim

The immune response due to Galα1,3-Galβ1-4GlcNAc-R(α-Gal) epitopes is an important factor in bioprosthetic heart valve failure. The aim of this study was to evaluate the immune reaction and anticalcification effect of α-galactosidase and decellularization for glutaraldehyde (GA)/genipin fixed bovine pericardium using α1,3-galactosyltransferase knockout(α-Gal KO) mouse(C57BL/6). Bovine pericardi...

2017
A Schmitt R Csiki A Tron B Saldamli J Tübel K Florian S Siebenlist E Balmayor R Burgkart

BACKGROUND The idea of tissue decellularization to gain matrices for tissue engineering is promising. The aim of the present study is to establish a safe and reproducible protocol for solid tissue decellularization that prevents the architecture of the matrix with the inherent vascular network. METHODS The study was performed in rat kidneys which were decellularized by a SDS-based perfusion p...

Journal: :Acta biomaterialia 2012
Gabriel L Converse Matt Armstrong Rachael W Quinn Eric E Buse Michael L Cromwell Sara J Moriarty Gary K Lofland Stephen L Hilbert Richard A Hopkins

Decellularized allografts offer potential as heart valve substitutes and scaffolds for cell seeding. The effects of decellularization on the quasi-static and time-dependent mechanical behavior of the pulmonary valve leaflet under biaxial loading conditions have not previously been reported in the literature. In the current study, the stress-strain, relaxation and creep behaviors of the ovine pu...

2016
Samantha L. Wilson Laura E. Sidney Siobhán E. Dunphy Harminder S. Dua Andrew Hopkinson

BACKGROUND There is a clinical need for biomimetic corneas that are as effective, preferably superior, to cadaveric donor tissue. Decellularized tissues are advantageous compared to synthetic or semi-synthetic engineered tissues in that the native matrix ultrastructure and intrinsic biological cues including growth factors, cytokines and glycosaminoglycans may be retained. However, there is cur...

Journal: :Biomaterials 2006
Erwin Rieder Anneliese Nigisch Barbara Dekan Marie-Theres Kasimir Ferdinand Mühlbacher Ernst Wolner Paul Simon Günter Weigel

Supporting structures derived from biological tissue have been used in numerous tissue-engineering applications. This study focuses on the immune response of human leukocytes toward decellularized or glutaraldehyde (GA) cross-linked vascular tissue in vitro. Porcine and human pulmonary roots were sterilized with antibiotics, decellularized or cross-linked with GA. Proteins of the vascular tissu...

Journal: :American journal of physiology. Heart and circulatory physiology 2005
Sylvain Roy Paolo Silacci Nikolaos Stergiopulos

To analyze the effects of decellularization on the biomechanical properties of porcine common carotid arteries, decellularization was performed by a detergent-enzymatic procedure that preserves extracellular matrix scaffold. Internal diameter, external diameter, and wall thickness were measured by optical microscopy on neighboring histological sections before and after decellularization. Ruptur...

2017
Arne A N Bruyneel Carolyn A Carr

Decellularization offers great potential to the field of tissue engineering, as this method gives rise to scaffold material with the native organ architecture by removing all cellular material and leaving much of the extracellular matrix (ECM) intact. However, many parameters may affect decellularization efficacy and ECM retention and, therefore, decellularization protocols need to be optimized...

2017
Hamid Tebyanian Ali Karami Ebrahim Motavallian Jafar Aslani Ali Samadikuchaksaraei Babak Arjmand Mohammad Reza Nourani

BACKGROUND Lung disease is the most common cause of death in the world. The last stage of pulmonary diseases is lung transplantation. Limitation and shortage of donor organs cause to appear tissue engineering field. Decellularization is a hope for producing intact ECM in the development of engineered organs. AIM The goal of the decellularization process is to remove cellular and nuclear mater...

Journal: :Plastic and reconstructive surgery 2015
Giorgio Giatsidis Erica Dalla Venezia Diego De Stefani Rosario Rizzuto Franco Bassetto

Introduction: Decellularization of tissues provides inductive extra-cellular matrices (ECM) for effective organ reconstruction: this promising approach has not been translated to breast reconstruction yet. 1, 2 We investigated the effectiveness of different decellularization protocols applied to porcine mammary glands with the purpose of developing an inductive biological matrix for prospective...

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