Degenerate primers-based polymerase chain reaction (PCR) are commonly used for isolation of unidentified gene sequences in related organisms. For designing the degenerate primers, we propose the use of local alignment search method for searching the conserved regions long enough to design an acceptable primer pair. To test this method, a WD40 repeat-containing domain protein from Beauveria bass...

It is well known that in operations research‎, ‎degeneracy can cause a cycle in a network‎ ‎simplex algorithm which can be prevented by maintaining strong‎ ‎feasible bases in each pivot‎. ‎Also‎, ‎in a network consists of n arcs‎ ‎and m nodes‎, ‎not considering any new conditions on the entering‎ ‎variable‎, ‎the upper bound of consecutive degenerate pivots is equal‎ $left( ‎begin{array}{c}‎ ‎n...

statement of problem: t-loop is one of the most popular closing loops in orthodontic profession. however its activation is in the contrary to bouschinger effect theory, since the formed bends would be opened during activation. the purpose of this study was to add a helix in a way to be more respondent to the bouschinger effect theory. objectives: to compare the load deflection, maximal elastic ...

The objective of the study in this paper is to define M₁,M₂-direction curves and M₁,M₂-donor curves of the spacelike curve γ via the Bishop frame of type-2 in E₁³. We obtained the necessary and sufficient conditions when the associated curves to be slant helices and general helices via the Bishop frame of type-2 in E₁³. After defining the spherical indicatrices of the associated curves, we obta...

Background: Androgen receptor (AR) plays a critical role in prostate cancer and male sexual differentiation.Mechanisms by which AR acts and regulations of AR nucleocytoplasmic shuttling are not understood well. Materials and Methods: Degenerate PCR and RACE Cloning of AR Gene; Phylogenetic Analysis and Molecular Modeling;Real-time Fluorescent Quantitative RT-PCR; Northern Blot Hybridization;In ...

In the present study we attempted to correlate the structure and function of the cry11a (72 kDa) and cry4a (135 kDa) proteins of Bacillus thuringiensis var israelensis. Homology modeling and secondary structure predictions were done to locate most probable regions for finding helices or strands in these proteins. The JPRED (JPRED consensus secondary structure prediction server) secondary struct...

Carbon nanoscrolls (CNSs) with tubular structure similar to the open multiwall carbonnanotube have been of hot debate during recent years. Due to its unique property, Graphene Nanoscroll (GNS) have attracted many research groups’ attention and have been used by them. They specially studied on energy storage devices such as batteries and super capacitors. These devices can be schematically...