Glucose 6-phosphate dehydrogenase (G6PD) from Streptomyces aureofaciens was purified and denatured in 6 M urea. Denaturation led to complete dissociation of the enzyme into its inactive monomers, 98% loss of the enzyme activity, about 30% decrease in the protein fluorescence and a 10 nm red shift in the emission maximum. Dilution of urea-denatured enzyme resulted in regaining of the enzyme acti...

European Train Control System (ETCS) is a signaling, control and train protection system that is replacing the national, incompatible safety systems within Europe. ETCS consists of the on-board subsystem (composed of ERTMS/ETCS on-board equipment, the on-board part of the GSM-R radio system and speci c transmission modules for existing national train control systems), and the track-side sub-sys...

We have studied hydration (and dehydration) of thermally denatured hen egg lysozyme using sorption calorimetry. Two different procedures of thermal denaturation of lysozyme were used. In the first procedure the protein was denatured in an aqueous solution at 90 degrees C, in the other procedure a sample that contained 20% of water was denatured at 150 degrees C. The protein denatured at 90 degr...

The development of procedures for fractionation of tissue deoxyribonucleate has received considerable attention since the demonstration (1, 2) of the compositional heterogeneity of such DNA. Earlier findings (3, 4) that the electrophoretic mobilities of native and denatured DNA were somewhat different suggested that zone electrophoresis might be used for separation of native and denatured DNA, ...

BACKGROUND Skin grafting with the preservation of denatured dermis is a novel strategy for the treatment of burn-injured skin. Denatured dermis has the ability to restore to the morphology and function of normal skin, but the underlying molecular mechanism is elusive. MicroRNAs (miRNA) are small noncoding RNAs and regulate normal physiology as well as disease development. In this study, we asse...