The rate of nitrous acid deamination of labeled cytosine residues in native Escherichia coli deoxyribonucleic acid was monitored in vitro by release of acid-soluble counts after treatment with uracil deoxyribonucleic acid glycosylase. The reaction exhibited a lag and was not stimulate by several agents previously shown to enhance base substitution mutagenesis during nitrous acid treatment of du...

Triostin antibiotics, which contain a cyclic peptide with a disulphide bridge, have been prepared by growing Streptomyces triostinicus in the presence of inorganic [3PS]sulphate. The labelled triostin A has been shown to behave in all respects similarly to the authentic natural product and to enable a much more sensitive radiochemical adaptation of the solvent-partition method for determining a...

The effects of the three antibiotics U-12,241, nogalamycin, and U-20,661 on (i) deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) synthesis in KB cell cultures and cell-free systems of bacterial and mammalian origin and on (ii) oxidative phosphorylation in rat liver mitochondria were compared. Nogalamycin and U-12,241 inhibited RNA synthesis more strongly than DNA synthesis in all test sys...

Deoxyribonucleic acid (DNA)-DNA hybridization on nitrocellulose filters can be used to assay for replication origin DNA from Escherichia coli if the DNA attached to the filters is enriched for the replication origin sequences. Such DNA can be readily isolated from very rapidly growing cells. When low amounts of this DNA were attached to filters, radioactively labeled DNA from the replication or...

The deoxyribonucleic acid of the T-even coliphages contains glucosylated and nonglucosylated 5-hydroxymethylcytosine in place of cytosine (l-6). In the case of deoxyribonucleic acid from phage T6, 72y0 of the 5-hydroxymethylcytosine residues are diglucosylated. In T2 deoxyribonucleic acid, 70% of the 5-hydroxymethylcytosine residues are monoglucosylated, but 5% bear diglucosyl units. A recent i...

Studies of deoxyribonucleic acid synthesis following inoculation with nucleo-polyhedrosis show a large burst of viral deoxyribonucleic acid synthesis late in the infection period.

Molecular beacon deoxyribonucleic acid computing is new research focus of intelligent control theory in recent years, it is also new bionic algorithm. It is well known that a very important problem how to determine or tune the proportional integral derivative controller parameters, because these parameters have a great influence on the stability and the performance of the control system. Parame...

The ability of different metal ions to promote transformation of Pseudomonas aeruginosa by deoxyribonucleic acid of the plasmid RP1 was examined. CaCl2, MgCl2, and MnCl2 were found to promote such transformation, although at different frequencies and with the optimum response at different concentrations. Only MgCl2 promoted transfection of P. aeruginosa by the linear deoxyribonucleic acid of ph...