Partial resistance of cell membranes to solubilization with mild detergents and the analysis of isolated detergent-resistant membranes (DRMs) have been used operationally to define membrane domains. Given the multitude of detergents used for this purpose, we sought to investigate whether extraction with different detergents might reflect the same underlying principle of domain formation. We the...

conclusions with the proper operation of wastewater the treatment plant and increasing the retention time, the removal efficiency of the detergents increased. in addition, according to the environmental standards for bod5, cod and the detergents, the results of the present study indicated that the outputs of these parameters from the sbr system were appropriate for agricultural irrigation. resu...

N-d-Gluco-N-methylalkanamide detergents have been synthesized. The detergents, which were produced in high yield and at low cost, compared favourably in biochemical studies with commonly used non-ionic detergents, including a chemically related n-alkyl glucoside. The ease of removal by dialysis, high solubilizing power and non-denaturing properties of this new class of detergents make them valu...

MICROCALORIMETRY Introduction Many industrial and biochemical applications depend on the usage of detergents and other surfactants. These compounds are indispensable, in particular, for the extraction, purification, and handling of membrane proteins. Owing to their amphiphilic nature, detergents can provide a membrane-mimetic environment required by integral membrane proteins to retain their na...

The solubilizing power of various nonionic and zwitterionic detergents as membrane protein solubilizers for two-dimensional electrophoresis was investigated. Human red blood cell ghosts and Arabidopsis thaliana leaf membrane proteins were used as model systems. Efficient detergents could be found in each class, i.e. with oligooxyethylene, sugar or sulfobetaine polar heads. Among the commerciall...

An optimization and comparison of trypsin digestion strategies for peptide/protein identifications by microLC-MS/MS with or without MS compatible detergents in mixed organic-aqueous and aqueous systems was carried out in this study. We determine that adding MS-compatible detergents to proteolytic digestion protocols dramatically increases peptide and protein identifications in complex protein m...

As anyone who has ever blown bubbles as or with a child knows, if you stop blowing before the sphere is complete, it collapses and disappears. The gas exchange surfaces of our lungs, the alveoli, are also highly curved, thin-walled, moist membranes that are open to the atmosphere. They too, would tend to go poof! and disappear, save for a mixture of phospholipids and proteins that lines the epi...