The release of cytochrome c from mitochondria is a crucial step in apoptosis, resulting in the activation of the caspase proteases. A further consequence of cytochrome c release is the enhanced mitochondrial production of superoxide radicals (O2.), which are converted to hydrogen peroxide by manganese-superoxide dismutase. Recently, we showed that cytochrome c is a potent catalyst of 2',7'-dich...

The fluorescent probes dichlorofluorescin (DCFH), dihydrorhodamine (DHR), and hydroethidine (HE) allow convenient assay of alveolar macrophage (AM) oxidant responses to enviromental particulates and pathogens. We sought to more precisely define the relationship of these measures of oxidant stress to production of pro-inflammatory cytokines. Normal AMs were challenged in vitro with a panel of so...

In this study, cell death regulation and induction in AML line from a relapsed MLL-rearranged model (MOLM-13) was investigated with doxorubin (Dox) betulinic acid (BetA), singly combination. CyQUANT Direct® Annexin V/propidium iodide double staining were used to measure the cytotoxic effects of compounds, respectively. Reactive oxygen species (ROS) generation measured using 2′,7′-dichlorofluore...

Localization and staining features of the oxidant-sensitive fluorescent probe 2'7'-dichlorofluorescin (DCFH) were evaluated in isolated cardiac muscle cells. Cardiomyocytes rapidly accumulated the probe and retained steady levels of DCFH and its highly fluorescent oxidized product dichlorofluorescein (DCF) in probe-free medium for 1.5 h. DCF was associated with mitochondria and was released by ...

In this study, various laboratory and field tests were performed to develop an effective automated particle-bound ROS sampling-analysis system. The system uses 2' 7'-dichlorofluorescin (DCFH) fluorescence method as a nonspecific, general indicator of the particle-bound ROS. A sharp-cut cyclone and a particle-into-liquid sampler (PILS) were used to collect PM(2.5) atmospheric particles into slur...

Introduction: Anti-inflammatory drugs are being utilized to treat cancer because of its inflammatory microenvironment. Objective: The objective this study is investigate the antioxidant potential indomethacin and genotoxicity, since free or loaded in polymeric nanocapsules using MCF-7 (human breast cancer) cells as an vitro model. Method: Development indomethacin-loaded polyepsiloncaprolactone ...

PURPOSE To investigate the protective effect of clusterin on oxidative stress-induced cell death of human corneal endothelial cells. METHODS Human corneal endothelial cells (HCECs) were cultured according to previously published methods. With treatment of various concentrations (0-50 mM) of tert-butyl hydroperoxide (tBHP) or clusterin, reactive oxygen species (ROS) producrion was measured usi...