We present a novel and efficient nanoporous microfluidic biochip consisting of a functionalized chitosan/anatase titanium dioxide nanoparticles (antTiO2-CH) electrode integrated in a polydimethylsiloxane (PDMS) microchannel assembly. The electrode surface can be enzyme functionalized depending on the application. We studied in detail cholesterol sensing using the cholesterol esterase (ChEt) and...

Microfluidic bioartificial organs allow the reproduction of in vivo-like properties such as cell culture in a 3D dynamical micro environment. In this work, we established a method and a protocol for performing a toxicogenomic analysis of HepG2/C3A cultivated in a microfluidic biochip. Transcriptomic and proteomic analyses have shown the induction of the NRF2 pathway and the related drug metabol...

Progressive research on design automation of digital microfluidic biochip is helping biochips to emerge as an effective alternative for several pathological experiments in the laboratories. This is often deployed for multiplexing several bioassays under optimized space and time constraints. We refer the droplets associated with bioassay of sample or reagents as serviceable droplets. During thes...

Microfluidic biochips are an alternative to conventional biochemical laboratories, and are able to integrate on-chip all the necessary functions for biochemical analysis. The " digital " biochips are manipulating liquids not as a continuous flow, but as discrete droplets on a two-dimensional array of electrodes. The main objective of this thesis is to develop top-down synthesis techniques for d...

Neisseria meningitidis (N. meningitidis), Streptococcus pneumoniae (S. pneumoniae), and Haemophilus influenzae type b (Hib) are three most common pathogens accounting for most bacterial meningitis, a serious global infectious disease with high fatality, especially in developing nations. Because the treatment and antibiotics differ among each type, the identification of the exact bacteria causin...

Blood typing is the most important test for both transfusion recipients and blood donors. In this paper, a low cost disposable blood typing integrated microfluidic biochip has been designed, fabricated and characterized. In the biochip, flow splitting microchannels, chaotic micromixers, reaction microchambers and detection microfilters are fully integrated. The loaded sample blood can be divide...

Microfluidics-based biochips, also referred to as lab-on-a-chip (LoC), are devices that integrate fluid-handling functions such as sample preparation, analysis, separation, and detection. This emerging technology combines electronics with biology to open new application areas including point-of-care diagnosis, on-chip DNA analysis, and automated drug discovery. As digital microfluidic biochips ...

Abstract Cyberphysical microelectrode-dot-array (CP-MEDA)-based digital microfluidic biochip (DMFB) is attracting more attention than its predecessor of traditional DMFB. Conventional DMFBs are mostly unable to recover from the errors incurred at assay run time, and thus, it leads unacceptable results. Recent studies have revealed vulnerability CP-DFMBs detect malicious intrusions during design...