agronomic and physiological traits and protein expression were compared between non-bt glandless upland cotton variety-zhong5629 and its bt-transgenic near-isogenic lines differing in cryiac gene. results showed that the bt isogenic line had higher plant height and rate of effective bolls, more internal bolls but lower yield, than the non-bt line. the bt isogenic line had lower chlorophyll cont...

The challenge associated with the use of synovial fluid for osteoarthritic proteome studies is optimization step, which involves depletion high abundant proteins from samples. objective this study was to develop a cost efficient and effective method remove albumin canine studies. Pooled samples were obtained clinically healthy dogs (n=5), no radiographic features osteoarthritis. acetone precipi...

Two-dimensional (2D) polyacrylamide gel electrophoresis is a classical technique for the separation of proteins. It Rrst appeared in the mid-1970s but for a long time it only found limited applications. Recently it has enjoyed an impressive renaissance. The major reasons for this are the introduction of the immobilized pH gradient (IPG) strips and the development of analytical methods capable o...

We compared cellular proteins from normal human blood lymphocytes, monocytes, and granulocytes, using highresolution two-dimensional electrophoresis. The leukocytes were isolated from peripheral blood by centrifugation on density step gradients (yielding a fraction of purified granulocytes and a lymphocyte/monocyte mixture) and monocytes were subsequently separated from lymphocytes by virtue of...

Phloem exudate collected from hybrid poplar (Populus trichocarpa x Populus deltoides) was estimated to have more than 100 proteins, of which 48 were identified using LC-MS/MS. Comparative two-dimensional gel electrophoresis demonstrated that two phloem exudate proteins were significantly (P<0.05) upregulated 24 h after leaf wounding. These were identified as pop3/SP1 and a thaumatin-like protei...

Reductive methylation has been used to radioactively label crude-extract proteins with 3H or 14C. The procedure achieved good isotope incorporation and resolution of proteins on two-dimensional polyacrylamide gels. It allows high-precision comparison of tissue samples by double-labelling and should facilitate the study of tissue proteins by two-dimensional gel electrophoresis.