A pectinase producing fungus was isolated from compost soil. Morphological characteristics, microscopic observations and molecular characterization based on internal transcribed spacer (ITS) sequences led to its identification as Aspergillus niger (GenBank Accession Number: JX556221) and it was designated as Aspergillus niger strain K3. In the present study, the culture condition of the media w...

In the present study, media composition for high production of pectinase by Enterobacter sp. PSTB-1 in submerged fermentation was optimized using response surface methodology (RSM). Mango fruit processing industrial waste (MIW) was used as substrate (carbon source) as it contains high amount of pectin. Enterobacter sp. PSTB-1 used in present study has given pectin clear zone (PCZ) of 34 mm is h...

The boron in plant cell walls, which is water-insoluble and in the solid state, is solubilized by pectinase digestion to give a dimeric rhamnogalacturonan II-borate (dRG-II-B) complex. To clarify the nondestructive structure of boron present in plant cell walls (as represented by sugar beet fiber), we performed 192- and 96-MHz 11B solid state NMR measurements. The use of a high field magnet fre...

Pectinases have multiple nature and various forms which are necessary for the hydrolysis of pectin in several conventional industrial and natural processes. In this investigation fourteen fungal strains which isolated from agricultural soils ,were screened for pectinase activity. Pectinase activity were measured in vitro by assaying released reducing sugar from pectin as sole carbon source. Asp...

In this study, Aspergillus terreus NCFT4269.10 was selected for the biosynthesis of pectinase after screening from fifteen native fungal strains as the performance of A. terreus was best in pectin hydrolyzing activity (2.75 cm). Aiming at the cost effective fermentation, several agro-industrial residues such as, Pearl Millet (PM), Finger Millet (FM), Orange Peels (OP), Mustard oil Cake (MoC) an...

Fusarium oxysporum strain BM-201 was treated with ultraviolet (UV) radiation to obtain a high pectinase-producing strain. Mutant UV-10-41 was obtained and then treated by diethyl sulfate. Next, the mutant UV-diethyl sulfate-43 derived from UV-10-41 was selected as high pectinase-producing strain. Mutant UV-diethyl sulfate-43 was incubated on slant for 10 generations, demonstrating that the pect...

Genes acquired by horizontal transfer are increasingly being found in animal genomes. Understanding their origin and evolution requires knowledge about the phylogenetic relationships from both source and recipient organisms. We used RNASeq data and respective assembled transcript libraries to trace the evolutionary history of polygalacturonase (pectinase) genes in stick insects (Phasmatodea). B...

Pectinase was produced by solidstate fermentation utilizing coffee pulp with Aspergillus niger CFR 305. The exogenous pectinase contained 7539 ±70 U/g of enzyme activity. Further the pectinase enzyme was partially decolorized with activated carbon and 80 % recovery of enzyme activity was obtained. The optimum pH and temperature of the crude decolorized pectinase was 4.5 and 50°C respectively. T...

In this paper, the saccharification process of okara fiber with pectinase, xylanase and cellulase was investigated as a preliminary attempt to effectively utilize okara fiber. The solubilization of okara fiber was raised in proportion to the number of enzymes used; that is, by a single enzyme, two enzymes and three enzymes, in that order. The saccharification of okara fiber was much more comple...

In this work, the effect of particle size on the enzymatic hydrolysis of milled and sieved sugarcane bagasse (SCB) was studied. The enzymatic hydrolysis and fermentability of superfine ground SCB (SGP400) using an enzyme cocktail strategy were also explored. Particle size reduction improved the enzymatic hydrolysis. The highest glucose yield was 44.75%, which was obtained from SGP400. The enzym...