We have developed a light scattering technique based on differential measurement and polarization (differential light scattering, DLS) capable in principle of retrieving timing information with picosecond resolution without the need for fast electronics. DLS was applied to sonoluminescence, duplicating known results (sharp turnaround, self-similar collapse); the resolution was limited by intens...

Fundamental studies about differential limen (DL) for word-based speech rate variations in Japanese are described. In our previous study, the DLs are expressed by subtractive difference of mora duration. In this report, however, to fit the expression for various global speech rate, the DLs are expressed by variation ratio of mora duration. We carry out auditory tests with stimuli made by equall...

Polyribosome sedimentation velocity centrifugation can be used to identify differential regulation of the translation of mRNAs. However, ultracentrifugation presents practical limitations on the number of sedimentation velocity gradients that can be run simultaneously. A method for sedimentation velocity analysis of polyribosomes is presented that is based on low-speed centrifugation of sucrose...

A method of zonal centrifugation was developed which separates rat thoracic duct lymphocytes (TDL) mainly according to size. The validity of the fractionation method was supported by light microscope observations, Coulter Counter sizing, and in vivo and in vitro labeling of lymphocytes. The distributions of lysosomal acid hydrolases in TDL fractionated by zonal centrifugation are similar to the...

Mesophase separation has been identified in a polycation/anionic-nonionic mixed micelle system formed by the coacervation of poly(diallyldimethylammoniumchloride)/sodium dodecylsulfate-Triton X-100 in 0.40 M NaCl. The resultant dense, optically clear fluid was studied by turbidity, dynamic light scattering (DLS), and rheology. The presence of two diffusion modes in DLS points to microscopic het...

The technique described here is a fast and simple method of extracting chloroplast DNA (cpDNA). It overcomes the need for differential centrifugation using density gradients. The leaves do not have to be kept in the dark and lyophilized before extraction, but lyophilization is still possible. The chloroplasts are specifically lysed in a cell extract of leaves, using a non-ionic detergent. After...

Description Logics (DLs) are the formalism underlying the standard web ontology language OWL 2. DLs have formal semantics which are the basis for powerful reasoning services. In this paper, we introduce the basic notions of DLs and the techniques that realize subsumption—the fundamental reasoning service of DL systems. We discuss two reasoning methods for this service: the tableau method for ex...