Metabolic conversion and distribution of the products of 7,12-dimethylbenz(a)anthracene (DMBA) were analyzed in the mouse mammary cell transformation model in organ culture. In order to determine the levels of uptake of DMBA, the glands were exposed to the transforming dosage of the procarcinogen (7.8 microM, 20 microCi/ml) for 24 h, and the level of uptake was determined to be 8 x 10(4) cpm/mg...

10-Fluoro-7,12-dimethylbenz(a)anthracene (10-F-DMBA) is a more potent skin tumor initiator in SENCAR mice when compared with the parent hydrocarbon 7,12-dimethylbenz(a)anthracene (DMBA). To elucidate the mechanism for this difference, the covalent binding of these two hydrocarbons to the DNA of mouse epidermal cells in vivo and in vitro was compared. The quantity of 10-F-DMBA covalently bound t...

Abstract Dimethylbenzantracene is a compound that radical reactive, genotoxic, and immunotoxic. Black cumin seeds have antioxidative properties. The study aimed to determine the effect of black seed ethanolic extract as an antioxidant by observing secretion activity ROI NO peritoneal macrophages in Sprague Dawley (SD) rats induced DMBA. This controlled experiment used 30 male SD rats. test anim...

DMBA, 7,12-dimethylbenz[a]anthracene, is a widely studied polycyclic aromatic hydrocarbon that has long been recognized as a probable human carcinogen. It has been found that DMBA is phototoxic in bacteria as well as in animal or human cells and photomutagenic in Salmonella typhimurium strain TA102. This article tempts to explain the photochemistry and photomutagenicity mechanism. Light irradia...

Thirty-nine derivatives of 7,12-dimethylbenz(a)anthracene (DMBA) were tested for mutagenicity towards Salmonella typhimurium strain TA100 in the presence of activating enzymes. The compounds tested included four sets of 3,4-, 5,6-, 8,9-, and 10,11-diols: those of DMBA, its 7and 12-hydroxymethyl derivatives, and the 7,12-dihydroxymethyl derivative. Several phenolic derivatives and formyl and car...

Introduction: DMBA is a chemical carcinogen that induces carcinomas within few weeks of its application. We developed an experimental model carcinogenesis induced by dissolved in 0,5% paraffin oil (DMBA-PO), verifying the inhibitory effect carcinogenicity phenyl isothiocyanate (PhITC), phenethyl (PhnITC) and benzyl (BITC). Material Methods: For this, 88 hamsters were distributed into three grou...

Tumor-initiating properties of complete carcinogens such as 7,12-dimethylbenz(a)anthracene (DMBA) are well known but not the mechanism of DMBA-mediated tumor promotion. Our hypothesis is that interleukin (IL)-1alpha, an early proinflammatory cytokine that initiates a cascade of other cytokines and growth factors, is up-regulated by DMBA and contributes to inflammation and carcinogenesis. We fou...

The tumor suppressor protein p53 is a transcription factor that regulates apoptotic responses produced by genotoxic agents. Previous studies have reported that 7,12-dimethylbenz[a]anthracene (DMBA)-induced bone marrow toxicity is p53-dependent in vivo. Our laboratory has shown that DMBA-induced splenic immunosuppression is CYP1B1- and microsomal epoxide hydrolase (mEH)-dependent, demonstrating ...

Benzo(e)pyrene [B(e)P], a weakly carcinogenic polycyclic aromatic hydrocarbon, modifies tumor induction in mouse skin and the induction of mutation in mammalian cells by carcinogenic hydrocarbons. To determine how B(e)P alters the activation of the carcinogen 7,12-dimethylbenz(a)anthracene (DMBA) to DNA-binding metabolites, the hydrocarbon-DNA adducts formed in Syrian hamster embryo cell cultur...

We previously demonstrated that murine bone marrow stromal cells express high levels of cytochrome P4501B1 (CYP1B1) that metabolizes 7,12-dimethylbenza[a]anthracene (DMBA), and that DMBA activates the Ah receptor (AhR) in these cells in vitro. More recently, we reported that CYP1B1 is required for DMBA-induced lymphoblastoma formation in vivo. In this study, we addressed the hypothesis that bon...