Objectives When the nerve is injured near its entrance to the muscle belly, we cannot perform conventional methods. One useful method in such a situation is neurotization surgery. In this study, Bone marrow mesenchymal stem cells (BMSCs) implanted into the paralyzed muscle after neurotization surgery. These cells can stimulate axon growth and motor endplate formation, also prevent muscle atroph...

BACKGROUND/AIM Most of the previous investigators have used various types of media for the culture of nerve cells. In order to optimize the culture conditions, we compared the growth rate and amino acid consumption by two popular neuron models, rat PC12 and human SH-SY5Y, grown in DMEM or DMEM: Ham's F-12 (1:1): non-essential amino acids, supplemented with 10% fetal bovine serum (referred to DM...

The anticataract activity of Acorus calamus roots extract against induced hydrogen peroxide cataractogenesis was assessed in Goat lenses. The in vitro phase of study was performed on lenses from Goat eyes incubated for 52 h at 37oC in Dulbecco Modified Eagle Medium (DMEM) containing H2O2 (0.5 mM) (Group I), in DMEM containing H2O2 (0.5 mM) and methanolic extract of Acorus calamus (MEAC), (Group...

Macrophage is the immune phagocytic cell, playing variety of immunological and inflammatory reactions. The macrophage activation has been extensively studied in vitro using culture media like Dulbecco's modified Eagle’s medium (DMEM) Ham's F-12 (F-12) response to bacterial infection, tumor development, cytokines so on. In study during co-culture with EL-4 cells, we found different phenotypes J7...

A previous study from our laboratory demonstrated the effects of in vitro three-dimensional(3D)-Printed collagen scaffolds on maintenance cryopreserved patient-derived melanoma explants. However, it remains unknown precisely whether culture condition influences 3D-printed loaded with explant(3D-PCS-PDME). To optimize conditions 3D-PCS-PDME), were manufactured a 3DX bioprinter. The scaffold-on-f...

We aimed to evaluate the effect of activated platelet-rich plasma (PRP) on proliferation and chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BMSCs). Six mature male rabbits were included in this study. PRP was obtained by two-step centrifugation from whole blood, it using CaCl2 solution. BMSCs isolated proliferated marrow characterized flow cytometry. Passage 3 cultured...

The multipotency and anti-inflammatory effects of mesenchymal stem cells (MSCs) make them attractive for cell therapy in regenerative medicine. A large number of MSCs is required for efficient therapy owing to the low homing efficiency of MSCs to target sites. Furthermore, owing to limitations in obtaining sufficient amounts of MSCs, in vitro expansion of MSCs that preserves their differentiati...

EMT (epithelial-mesenchymal transition) is a key process in the development of liver fibrosis. This process is also essential for liver morphogenesis in embryonic development. To study the cellular and molecular basis of EMT, we established two phenotypically different SV40 large T antigen-immortalized cell lines from rat hepatocytes. The first cell line, which had an epithelial morphology and ...

The objective of this study was to test the hypothesis that human adipose tissue-derived adult stem cells (ASCs) can be effectively cryopreserved and stored in liquid nitrogen using a freezing medium containing a high-molecular-weight polymer, polyvinylpyrrolidone (PVP), as the cryoprotective agent (CPA) instead of dimethylsulfoxide (DMSO). To this end we investigated the postfreeze/thaw viabil...

It was previously shown that serum low density lipoprotein (LDL) binds to type I collagen gels and that the binding is increased after modification by cultured endothelial cells. It is now demonstrated that when LDL is incubated with cells cultured in Dulbecco's modified minimal essential medium (DMEM), the subsequent binding of LDL to collagen is considerably less than after incubation with en...