background: dna isolation procedure can significantly influence the quantification of dna by real time pcr specially when cell free dna (cfdna) is the subject. to assess the extraction efficiency, linearity of the extraction yield, presence of co-purified inhibitors and to avoid problems with fragment size relevant to cfdna, development of appropriate external dna control (edc) is challenging. ...

objective(s) this study planned to assess the value of pcr is6110 assay in tissue specimens of needle pleural biopsy in patients suspicious to pleural tuberculosis. materials and methods sixty eight patients with lymphocytic exudative pleural effusion underwent pleural biopsy. tissue samples were sent for pathologic examination and pcr is6110 assay. the results of pcr reported as positive/ nega...

three tuberculous, twenty-one non-tuberculous mycobacterial (ntm) reference strains and seventy two isolates classified by biochemical tests were shown to produce specific sets of dna fragments in a polymerase chain reaction with single universal primer (up-pcr). a rather wide limit of tolerance for variations in procedure of pcr mixture preparation and thermocycling parameters was found. there...

background and objective: to estimate the risk of human papilloma virus (hpv) infection for cervical malignancies, we conducted a case-control study in southern iran (hormozgan province). materials and methods: for this purpose,52 paraffin embedded blocks with exact diagnosis of cervical carcinoma(50 carcinomas and 2 carcinomas in situ) from 2001 to 2006 and 52 praffin embedded blocks of cervic...

detection of genetically modified organisms (gmos) in food is an important issue for all the subjects involved in food control and customer’s right. due to the increasing number of gmos imported to iran during the past few years, it has become necessary to screen the products in order to determine the identity of the consumed daily foodstuffs. in this study, following the extraction of genomic ...