bladder cancer is the most frequent genitourinary malignancy in iran. environmental and genetic factors are the two factors linked with bladder cancer expansion. the aim of this study was to investigate the role of pten gene and environmental risk factors on the progression and prognosis of bladder cancer.we evaluated 55 tumor specimens and 66 bladder mucosa samples of non-cancerous patients be...

Sequence variants of human mitochondrial DNA (mt DNA) have been implicated in a variety disorders and conditions. Massive parallel sequencing is becoming increasingly popular due to its efficiency cost-effectiveness. In relation acquiring significant sequence information like levels heteroplasmy mt DNA, it offers marked improvement compared previous methods used. Here we describe variant callin...

The nature and pace of genome mutation is largely unknown. Standard methods to investigate DNA-mutation rely on arraying or sequencing DNA from a population of cells, hence the genetic composition of individual cells is lost and de novo mutation in cell(s) is concealed within the bulk signal. We developed methods based on (SNP-) arraying and next-generation sequencing of single-cell whole-genom...