BACKGROUND Prognostic factors, including both histopathological and biochemical variables, influence the choice of modality and the course of therapy in breast cancer. The biomarkers found in biological fluids, particularly in blood, apparently hold the best promise for the development of screening assays. AIM To find out if any correlation exists between blood DNA level and tumor stage, size...

BACKGROUND AND OBJECTIVES DNA molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings. MATERIALS AND METHODS Here a PCR-supported procedure is described that uses 10 primer pairs targeting chromosomal DNA from the harmless vaccinal Bacillus anthracis Sterne 34F2 strain as template. A single PCR protocol is used to reproduce a...

background: indirect genetic diagnosis using polymorphic dna markers can detect carriers of hemophilia a. this technique is preferable in developing countries because of its simplicity and cost effectiveness compared to direct mutation analysis. in the present study, we examined usefulness of intragenic marker bcl i restriction fragment length polymorphism (rflp) at intron 18, for carrier detec...

the aim of this study was to determine the effect of marker density, level of heritability, number of qtls, and size of training set on the genomic accuracy over three generations. thereby, a trait was simulated with heritability of 0.10, 0.25 or 0.40. for each animal, a genome with 20 chromosomes, 1 morgan each, was simulated. different marker densities (2000, 4000 and 6000 markers) and 400 an...

The aim of this study was to determine the effect of marker density, level of heritability, number of QTLs, and size of training set on the genomic accuracy over three generations. Thereby, a trait was simulated with heritability of 0.10, 0.25 or 0.40. For each animal, a genome with 20 chromosomes, 1 Morgan each, was simulated. Different marker densities (2000, 4000 and 6000 markers) and 400 an...

The gapped duplex DNA approach to oligonucleotide-directed construction of mutations (Kramer et al. 1984, Nucl. Acids Res. 12, 9441-9456) has been developed further. A procedure is described that makes in vitro DNA polymerase/DNA ligase reactions dispensable. Direct transfection of host bacteria with gdDNA molecules of recombinant phage M13 plus mutagenic oligonucleotide results in marker yield...

This research aimed to assess the genetic polymorphism of BMP15 gene concerning sex hormone levels and birth type in Awassi ewes. The genome DNA 138 ewes was isolated (52 produced a singleton 86 twins). exon-2 amplifiable by polymerase chain reaction (PCR), so two genotypes were identified based on 141 bp amplicons: TT TA. A sequencing revealed novel mutation, c.50980646T>A, TA genotype. single...

Short tandem repeat (STR) typing has become the workhorse of modern forensic DNA analysis. The most common form of STR typing uses laser-induced fluorescence detection of dye-labeled polymerase chain reaction (PCR) products following capillary electrophoresis (CE) size-based separation. This unit describes the techniques and marker systems most widely used around the world in constructing crimi...