− Although liposomes have been applied as drug delivery systems in various fields, the usage was limited due to the low encapsulation efficiency compared to other carrier systems. Here, cationic liposomes were prepared by mixing 1,2-dioleoyl-3-trimethylammoniopropane (DOTAP) as a cationic lipid, 1,2-dioleoyl-sn-glycerol-phosphoethanolamine (DOPE) and cholesterol (CH), and the liposomes were hyd...

Objective: Transient expression of therapeutic genes within lung allografts may modulate the pathological processes following allotransplantation. Whilst ef®cient gene transfer to lungs has been reported with viral vectors, their usefulness is limited on the grounds of safety. Since non-viral systems overcome many of these safety issues, our studies were designed to evaluate the ef®ciency of se...

Multifunctional, lipopolyplex formulations comprising a mixture of cationic liposomes and cationic, receptor-targeting peptides have potential use in gene therapy applications. Lipopolyplex formulations described here are typically far more efficient transfection agents than binary lipoplex or polyplex formulations. It has been shown previously that the peptide component mediates both DNA packa...

Oligonucleotides able to hybridize bacterial RNA via in situ hybridization may potentially act as new antimicrobials, replacing antibiotics, and fast vivo diagnostic probes, outperforming current clinical methodologies. Nonetheless, oligonucleotides are not efficiently permeate the multi-layered envelope reach their target cytosol. Cationic fusogenic liposomes here suggested vehicles enable int...

background: dna vaccines are third generation vaccines which have made promises to combat infectious diseases. cationic liposomes are used as effective delivery systems for dna vaccines to generate stronger immunity. objective: encapsulation of pcdna3.1+pa plasmid, encoding protective antigen (pa) of bacillus anthracis (b. anthracis) into cationic liposomes, and evaluation of its effect on spec...

the development of efficient and safe carrier system to transfer dna into cells is essential in non-viral gene therapy. the aim of the present study was to evaluate the effect of linear polyetheneimine (lpei) (2500 da) on the physicochemical and biological properties of lipopolyplexes constructed from liposomes and lpei. materials and methods different lipopolymers were synthesized from lpei an...

Materials. DOTAP (1,2-dioleoyloxypropyl)-N,N,N-trimethylammonium chloride), DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) and cholesterol (Chol) were purchased from Avanti Polar lipids (USA). Solvents (chloroform and methanol) were obtained from Fisher Scientific (UK). Scrambled siRNA and apoptotic siPLK1 were purchased from Eurogentec (Southampton, UK). The fluorescence dye NIR-797 (λex...