Resonon utilizes line-scan spectral imaging, where a scene is limited to one dimension with a slit, and then imaged off a diffraction grating to obtain spectral information for each pixel along the slit. A second spatial dimension is obtained by scanning the object as multiple frames are collected. Here, electrophoresis was used as the scanning mechanism, as illustrated in Figures 1 and 2. Prot...

A two dimensional electrophoretic method is described for the routine clinical analysis of urinary proteins. Cellulose acetate electrophoresis is used for the first dimension, and SDS (sodium dodecyl sulphate) electrophoresis for the second dimension, the latter being performed together with gel staining (Coomassie Blue) on the „Phast System". The Separation media are supplied äs "ready-to-use"...

double*Speed divide (double*Length, double*Time); The original Java language specification defines: The signature of a method consists of the name of the method and the number and types of formal parameters to the method.. This definition does not change; hence a method signature does not contain dimension specifiers of the formal parameters. Instead the dimension specifiers constitute a rather...

One third of all genes of various organisms encode membrane proteins, emphasizing their crucial cellular role. However, due to their high hydrophobicity, membrane proteins demonstrate low solubility and a high tendency for aggregation. Indeed, conventional two-dimensional gel electrophoresis (2-DE), a powerful electrophoretic method for the separation of complex protein samples that applies iso...

The limited resolution of serum proteins achieved with a simplified technique of two-dimensional electrophoresis (Clin. Chim. Acta 103: 51-59, 1980) has been improved by using different ampholyte (Ampholine) mixtures in the first dimension, to obtain relatively shallow pH gradients, and prolonged electrophoresis time in the second dimension. The technique has been further simplified, without ne...

The mobilities of oligomers of phage lambda DNA and of yeast chromosomes in agarose gels during field inversion gel electrophoresis (FIGE) were measured at different pulse times and electric fields. Also the ratios between forward and backward pulse times and/or field gradients were varied. The problem of 'band inversion' during FIGE, leading to an ambiguity in the mobility of large DNA fragmen...

DNA is increasingly being used as an ideal material for the construction of nanoscale structures, circuits, and machines. Toehold-mediated DNA strand displacement reactions play a very important role in these enzyme-free constructions. In this study, the concept of metallo-toehold was utilized to further develop a mechanism for strand displacement driven by Ag+ ions, in which the intercalation ...

Microfabricated, plastic electrophoresis chips have been constructed on polycarbonate substrates. 2 cm long, entirely monolithic devices have been fabricated with channel cross sections of 20 m high and 50200 m wide. Successful separations of double stranded DNA have been performed in 0.5in 2-5 minutes with a resolution of 90,000 theoretical plates. With a substrate cost of 25 cents per blank p...