In order to produce doubled haploid lines of bread wheat resistant to strip or yellow rust, three F1 wheat hybrids were crossed using pollens of three maize hybrids. Out of 1071 pollinated florets, success in seed set ranged from 63.1% to 93.3% (mean 78%). Differences in seed set among the crosses were not significant. Embryo formation in the seeds developed on different crosses also varied fro...

The detection of meiotic crossovers in crop plants currently relies on scoring DNA markers in a segregating population or cytological visualization. We investigated the feasibility of using flow-sorted haploid nuclei, Phi29 DNA polymerase-based whole-genome-amplification (WGA) and multi-locus KASP-genotyping to measure meiotic crossovers in individual barley pollen grains. To demonstrate the pr...

Abstract Wheat is the most widely grown food crop, with 761 Mt produced globally in 2020. To meet expected grain demand by mid-century, wheat breeding strategies must continue to improve upon yield-advancing physiological traits, regardless of climate change impacts. Here, best performing doubled haploid (DH) crosses an increased canopy photosynthesis from field experiments literature were extr...

this study involves in vitro androgenesis of zea maysl. via anther culture. combination of two embryoinduction media (imss & ypm) in presence of different colchicine concentrations (0, 100, 200, 250, 300and 400 mg/l ) in the pretreatment medium (iml) andpretreatment duration (0, 3, 6 and 9 days) in twogenotypes (dh5×dh7 and eth-m82) were tested.after colchicine pretreatment, anthers were tr...

ninety barley doubled haploid lines originated from crossing between six barley cultivars in cereal research department, seed and plant improvement institute, karaj, iran were evaluated for resistance to powdery mildew and some agronomic traits in field in 2007. six parent cultivars and a powdery mildew susceptible check cultivar, afzal were also included. sixty two doubled haploid lines showed...

Doubled-haploid lines (DHs) are normally produced from F1 plants in maize (Zea mays L.). Several studies have found a low frequency of recombinants doubled haploids that could limit the selection response. Hence, an attempt was made to produce F2 generation verify whether one more round meiotic recombination lead increased genetic variability and assess response selection. The two cross-combina...

Production of doubled haploids (DHs) is an important methodology to speed the process of breeding and development of mapping populations in crops. The procedure for DH production includes two major steps: haploid induction and chromosome doubling. In recent years, wide hybridization between wheat and maize has become a main approach for haploid production in wheat. In this method, the maize chr...

In vivo doubled haploid (DH) production based on crossing heterozygous germplasm with mtl inducer lines promises to transform modern rice (Oryza sativa) breeding. However, this technology is limited, as inducers and pollen acceptors have asynchronous heading dates. To address obstacle, we developed a panel of multiple-heading-date that produce for more than 35 days. We edited the MTL gene in hy...