Polarized FTIR ATR spectroscopy was used to investigate the interaction of mitochondrial creatine kinase (Mi-CK) and intestinal alkaline phosphatase (AP) with model membrane assemblies. Mi-CK was immobilized by adsorption to the negatively charged cardiolipin (CL) leaflet of a supported CL/DPPA bilayer. H-D-exchange of the enzyme and the stability under flowthrough conditions of the protein/mem...

Treatment of bis(diphenylphosphino)amine (dppa) with (dimethylsulfide)gold(I) chloride in the molar ratio 1:2 leads to the dinuclear complex (dppa)Au2Cl2 in 95% yield. The compound was crystallized from diethyl ether/dimethylformamide as the 1:1 solvate with dmf, the crystal struc­ ture of which has been determined. The complex features an intramolecular Au—Au contact [3.121(1) A] and an interm...

Abstract A diverse range of ureas, N-acylureas, carbamates, and thiocarbamates has been synthesized in good to excellent yields by reacting N-acylbenzotriazoles individually with amines or amides phenols thiols the presence diphenylphosphoryl azide (DPPA) as a suitable donor anhydrous toluene at 110 °C for 3–4 hours. In this route, DPPA was found be alternative trimethylsilyl sodium Curtius deg...

Bacteriocin AS-48 produced by Enterococcus faecalis S-48 is a ribosomally synthesized cyclic peptide (7.4 kDa) of broad inhibitory spectrum against Gram-positive and Gram-negative bacteria. Simple monolayers of AS-48 and of dipalmitoyl phosphatidic acid (DPPA) at the air-water interface are studied. The AS-48 interfacial behavior in the function of pH explains the biological activity of the pep...

The influence of hormones in water subphase on the stability of monolayers built of phospholipid mixtures extracted from embryogenic (PLE) and nonembryogenic (PLNE) wheat calli was examined. Additionally, experiments on individual lipids, dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidic acid (DPPA), were performed. DPPC was chosen because it was the main phospholipid present in...

The interaction of DPPA which is an important component in cellular membrane with molecules of water asmedia, cause some changes in geometry of DPPA .In this study we focused our attention on comparing thismolecule with related hydrated complexes employing different basis sets at hartree-fock levels of theory. Thetheoretical NMR shielding tensor of some imported atoms were also reported.

Phase-sensitive vibrational sum frequency generation is employed to investigate the water structure at phospholipid/water interfaces. Interfacial water molecules are oriented preferentially by the electrostatic potential imposed by the phospholipids and have, on average, their dipole pointing toward the phospholipid tails for all phospholipids studied, dipalmitoyl phosphocholine (DPPC), dipalmi...

the interaction of dppa which is an important component in cellular membrane with molecules of water asmedia, cause some changes in geometry of dppa .in this study we focused our attention on comparing thismolecule with related hydrated complexes employing different basis sets at hartree-fock levels of theory. thetheoretical nmr shielding tensor of some imported atoms were also reported.

9 10 Abstract 11 12 Polarization modulation infrared reflection spectra of a 1,2-dipalmitoyl-sn-glycero-3-phosphatidic acid (DPPA) monolayer on a subphase 13 containing 5 mM tetracycline hydrochloride (TC) were collected under varying surface pressures at the air–water interface. Statistical 14 correlation spectroscopy using 2D IR, bn and kn correlation analyses were performed on these spectra ...

Despite research into the nutritional requirements of Helicobacter pylori, little is known regarding its use of complex substrates, such as peptides. Analysis of genome sequences revealed putative ABC-type transporter genes for dipeptide (dppABCDF) and oligopeptide (oppABCD) transport. Genes from each system were PCR amplified, cloned, and disrupted by cassette insertion either individually (dp...